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A betabaculovirus DNA polymerase cannot substitute for the DNA polymerase of the alphabaculovirus Autographa californica nucleopolyhedrovirus

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Abstract

DNA polymerase (DNApol) is present in all baculoviruses and plays a crucial role in viral DNA replication. Previously we showed that the DNApol of the alphabaculovirus group II Spodoptera litura nucleopolyhedrovirus (SpltNPV) could partially substitute for the DNApol of a group I alphabaculovirus, Autographa californica multiple nucleopolyhedrovirus (AcMNPV). However, it is not known if a betabaculovirus DNApol could subsititute for the alphabaculovirus DNApol in AcMNPV. In this report, DNApol of the betabaculovirus Pieris rapae granulovirus (PiraGV) was inserted into a dnapol-null AcMNPV bacmid, creating Bac-AcΔpol:PrPol. The repair virus did not spread to neighboring cells; virus growth curve and real-time PCR revealed that the PiraGV dnapol substitution abrogated AcMNPV DNA replication and virus production. Immunofluorescence microscopy showed that PiraGV DNApol could be expressed and localized to the nucleus. Collectively, our results suggested that the alphabaculovirus AcMNPV DNApol could not be replaced by a DNApol from the betabaculovirus, PiraGV.

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Acknowledgements

We thank Professor Baoshan Chen (College of Life Science and Technology, Guangxi University) for his generous gift of the PiraGV genome DNA used in this study.

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Author notes

  1. Guoqing Chen and Yang Fang contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 311400, China

    Guoqing Chen, Yang Fang, Lijuan Wu & Guozhong Feng

  2. Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada

    Peter J. Krell

  3. China National Rice Research Institute, Hangzhou, 311400, China

    Qing Yan

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  1. Guoqing Chen
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  2. Yang Fang
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  3. Lijuan Wu
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  4. Qing Yan
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Corresponding author

Correspondence to Guozhong Feng.

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Funding

This research was supported by a National Natural Science Foundation of China (No. 31572006), incremental project of Chinese Academy of Agricultural Sciences (No. 2015ZL058), innovation project of Chinese Academy of Agricultural Sciences, and the Central Level Public Interest Research Institute for Basic R & D Special Fund Business (Nos. 2013RG001-2, 2014RG002-3).

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All the authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Chen, G., Fang, Y., Wu, L. et al. A betabaculovirus DNA polymerase cannot substitute for the DNA polymerase of the alphabaculovirus Autographa californica nucleopolyhedrovirus. Arch Virol 162, 3487–3492 (2017). https://doi.org/10.1007/s00705-017-3468-0

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  • DOI: https://doi.org/10.1007/s00705-017-3468-0

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