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Characterization of siRNAs derived from cucumber green mottle mosaic virus in infected cucumber plants

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Abstract

Virus-derived small interfering RNAs (vsiRNAs) of cucumber green mottle mosaic virus (CGMMV), a member of the genus Tobamovirus, were characterised in cucumber plants by deep sequencing. CGMMV vsiRNAs of 21-22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CGMMV-infected cucumber plants. The 5′-terminal base of vsiRNAs was biased towards C/A/U, suggesting that CGMMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were also predicted.

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Acknowledgment

This research was financially supported by the support program of General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China (201310068), National Natural Science Foundation of China (Grant No. 31401711) and a grant from Beijing University of Agriculture (BNRC&YX201415).

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Authors and Affiliations

  1. College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China

    Yongqiang Li & Qiaoxia Shang

  2. Plant Laboratory of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 101312, China

    Congliang Deng, Xiaoli Zhao & Xingliang Liu

  3. Chinese Society of Inspection and Quarantine, Beijing, 100029, China

    Qi Zhou

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  1. Yongqiang Li
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  2. Congliang Deng
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  3. Qiaoxia Shang
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  4. Xiaoli Zhao
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  6. Qi Zhou
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Correspondence to Yongqiang Li or Qi Zhou.

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Li, Y., Deng, C., Shang, Q. et al. Characterization of siRNAs derived from cucumber green mottle mosaic virus in infected cucumber plants. Arch Virol 161, 455–458 (2016). https://doi.org/10.1007/s00705-015-2687-5

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  • DOI: https://doi.org/10.1007/s00705-015-2687-5

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