Skip to main content
SpringerLink
Log in

Occurrence and characterization of plum pox virus strain D isolates from European Russia and Crimea

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Numerous plum pox virus (PPV) strain D isolates have been found in geographically distant regions of European Russia and the Crimean peninsula on different stone fruit hosts. Phylogenetic analysis of their partial and complete genomes suggests multiple introductions of PPV-D into Russia. Distinct natural isolates from Prunus tomentosa were found to bear unique amino acid substitutions in the N-terminus of the coat protein (CP) that may contribute to the adaptation of PPV-D to this host. Serological analysis using the PPV-D-specific monoclonal antibody 4DG5 provided further evidence that mutations at positions 58 and 59 of the CP are crucial for antibody binding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Cambra M, Asensio M, Gorris MT, Perez E, Camarasa E, Garcia JA, Moya JJ, Lopez-Abella D, Vela C, Sanz A (1994) Detection of plum pox potyvirus using monoclonal antibodies to structural and non-structural proteins. EPPO Bull 24:569–577

    Article  Google Scholar 

  2. Cambra M, Capote N, Myrta A, Llacer G (2006) Plum pox virus and the estimated costs associated with sharka disease. EPPO Bull 36:202–204

    Article  Google Scholar 

  3. Candresse T, Cambra M, Dallot S, Lanneau M, Asensio M, Gorris MT, Revers F, Macquaire G, Olmos A, Boscia D, Quiot JB, Dunez J (1998) Comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the D and M serotypes of plum pox potyvirus. Phytopathology 88:198–204

    Article  CAS  PubMed  Google Scholar 

  4. Candresse T, Saenz P, Garcia JA, Boscia D, Navratil M, Gorris MT, Cambra M (2011) Analysis of the epitope structure of Plum pox virus coat protein. Phytopathology 101:611–619

    Article  CAS  PubMed  Google Scholar 

  5. Carbonell A, Maliogka VI, de Jesus Perez J, Salvador B, San Leon D, Garcia JA, Simon-Mateo C (2013) Diverse amino acid changes at specific positions in the N-terminal region of the coat protein allow Plum pox virus to adapt to new hosts. Mol Plant Microbe Interact 26:1211–1224

    Article  CAS  PubMed  Google Scholar 

  6. Chirkov S, Ivanov P, Sheveleva A (2013) Detection and partial molecular characterization of atypical plum pox virus isolates from naturally infected sour cherry. Arch Virol 158:1383–1387

    Article  CAS  PubMed  Google Scholar 

  7. Chirkov S, Prihodko Y, Ivanov P, Sheveleva A (2015) Population of Plum pox virus in European Russia seems to be the most diverse in the world. In: XVIII international plant protection congress, Berlin, p 349

  8. Damsteegt VD, Waterworth HE, Mink GI, Howell WE, Levy L (1997) Prunus tomentosa as a diagnostic host for detection of Plum pox virus and other Prunus viruses. Plant Dis 81:329–332

    Article  Google Scholar 

  9. Dolja VV, Haldeman-Cahill R, Montgomery AE, Vandenbosch KA, Carrington JC (1995) Capsid protein determinants involved in cell-to-cell and long distance movement of tobacco etch potyvirus. Virology 206:1007–1016

    Article  CAS  PubMed  Google Scholar 

  10. Garcia JA, Glasa M, Cambra M, Candresse T (2014) Plum pox virus and sharka: a model potyvirus and a major disease. Mol Plant Pathol 15:226–241

    Article  CAS  PubMed  Google Scholar 

  11. Glasa M, Prikhodko Y, Predajna L, Nagyova A, Shneyder Y, Zhivaeva T, Šubr Z, Cambra M, Candresse T (2013) Characterization of sour cherry isolates of Plum pox virus from the Volga basin in Russia reveals a new cherry strain of the virus. Phytopathology 103:972–979

    Article  CAS  PubMed  Google Scholar 

  12. James D, Varga A (2004) Preliminary molecular characterization of Plum pox virus isolate W3174: evidence of a new strain. Acta Hortic 657:177–182

    Article  CAS  Google Scholar 

  13. James D, Varga A, Sanderson D (2013) Genetic diversity of Plum pox virus: strains, diseases and related challenges for control. Can J Plant Pathol 35:431–441

    Article  Google Scholar 

  14. James D, Sanderson D, Varga A, Greig N, Stobbs LW (2015) Analysis of the genetic diversity and relationships of selected Canadian isolates of Plum pox virus. Acta Hortic 1063:33–40

    Article  Google Scholar 

  15. Maejima K, Himeno M, Komatsu K, Takinami Y, Hashimoto M, Takahashi S, Yamaji Y, Oshima K, Namba S (2011) Molecular epidemiology of Plum pox virus in Japan. Phytopathology 101:567–574

    Article  PubMed  Google Scholar 

  16. Matic S, Al Rwahnih M, Myrta A (2006) Diversity of Plum pox virus isolates in Bosnia and Herzegovina. Plant Pathol 55:11–17

    Article  CAS  Google Scholar 

  17. Matic S, Elmaghraby I, Law V, Varga A, Reed C, Myrta A, James D (2011) Serological and molecular characterization of isolates of Plum pox virus strain El Amar to better understand its diversity, evolution and unique geographical distribution. J Plant Pathol 93:303–310

    CAS  Google Scholar 

  18. Nemchinov L, Hadidi A (1998) Specific oligonucleotide primers for the direct detection of plum pox potyvirus-cherry subgroup. J Virol Methods 70:231–234

    Article  CAS  PubMed  Google Scholar 

  19. Olmos A, Cambra M, Dasi MA, Candresse T, Esteban O, Gorris MT, Asensio M (1997) Simultaneous detection and typing of Plum pox potyvirus (PPV) isolates by hemi-nested PCR and PCR-ELISA. J Virol Methods 68:127–137

    Article  CAS  PubMed  Google Scholar 

  20. Revers F, Garcia JA (2015) Molecular biology of potyviruses. Adv Virus Res 92:101–199

    Article  PubMed  Google Scholar 

  21. Schneider WL, Damsteegt VD, Gildow FE, Stone AL, Sherman DJ, Levy LE, Mavrodieva V, Richwine N, Welliver R, Luster DG (2011) Molecular, ultrastructural, and biological characterization of Pennsylvania isolates of Plum pox virus. Phytopathology 101:627–636

    Article  PubMed  Google Scholar 

  22. Sheveleva A, Ivanov P, Chirkov S, Prihodko Y, Varga A, James D (2012) Plum pox virus W appears to be the most variable strain of the seven recognized strains of the virus. Petria 22:226–232

    Google Scholar 

  23. Sheveleva A, Kudryavtseva A, Speranskaya A, Belenikin M, Melnikova N, Chirkov S (2013) Complete genome sequence of a novel Plum pox virus strain W isolate determined by 454 pyrosequencing. Virus Genes 47:385–388

    Article  CAS  PubMed  Google Scholar 

  24. Šubr Z, Pittnerova S, Glasa M (2004) A simplified RT-PCR-based detection of recombinant Plum pox virus isolates. Acta Virol 48:173–176

    PubMed  Google Scholar 

  25. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  26. Wallis CM, Stone AL, Sherman DJ, Damsteegt VD, Gildow FE, Schneider WL (2007) Adaptation of plum pox virus to a herbaceous host (Pisum sativum) following serial passages. J Gen Virol 88:2839–2845

    Article  CAS  PubMed  Google Scholar 

  27. Weber PH, Bujarski JJ (2015) Multiple functions of capsid proteins in (+) stranded RNA viruses during plant-virus interactions. Virus Res 196:140–149

    Article  CAS  PubMed  Google Scholar 

  28. Wetzel T, Candresse T, Macquaire G, Ravelonandro M, Dunez J (1992) A highly sensitive immunocapture polymerase chain reaction method for Plum pox virus detection. J Virol Methods 39:27–37

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We gratefully acknowledge Drs. Anatoly Yushev and Olga Radchenko (Vavilov Research Institute of Plant Industry) for their cooperation in the Pavlovsk Research Station stone fruit collection survey and for helpful discussions. We thank Dr. Delano James for the serological analysis of the isolate Vulcan. This work was supported by the Russian Scientific Foundation (research project no. 14-24-00007).

Author information

Authors and Affiliations

  1. Lomonosov Moscow State University, Moscow, Russia

    Sergei Chirkov, Peter Ivanov & Anna Sheveleva

  2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia

    Anna Kudryavtseva

  3. All-Russian Center of Plant Quarantine, Moscow, Russia

    Yuri Prikhodko

  4. Nikita Botanical Gardens, National Scientific Center, Yalta, Russia

    Irina Mitrofanova

Authors
  1. Sergei Chirkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peter Ivanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anna Sheveleva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Anna Kudryavtseva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yuri Prikhodko
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Irina Mitrofanova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sergei Chirkov.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Fig. S1 Geographical localities (red circles) of plum pox virus isolates studied in this work. (DOC 257 kb)

705_2015_2658_MOESM2_ESM.doc

Fig. S2 Offset in-frame triplet deletion in the NIb gene of the isolate Cr11. Nucleotide and deduced amino acid sequences of the isolates K9 and Cr11 around the deletion site are shown. Numerals above alignment show nucleotide positions in the complete genome of the isolate K9. The ?aspartic? acid residue deleted in the Cr11 polyprotein is shaded. (DOC 26 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chirkov, S., Ivanov, P., Sheveleva, A. et al. Occurrence and characterization of plum pox virus strain D isolates from European Russia and Crimea. Arch Virol 161, 425–430 (2016). https://doi.org/10.1007/s00705-015-2658-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-015-2658-x

Keywords

Search

Navigation