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Clinical and molecular epidemiology of norovirus infection in adults with acute gastroenteritis in Ji’nan, China

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Abstract

Acute gastroenteritis caused by human noroviruses (NoVs) has become an important public health problem worldwide. This study was carried out to investigate the rates of NoV infections and the genetic characteristics of NoVs in adult outpatients with acute gastroenteritis in Ji’nan, a large eastern city in China. A total of 480 fecal samples were collected from outpatients at the Shandong University Qilu Hospital between June 2010 and May 2011. Of the collected samples, 42 (42/480, 8.75 %) were positive for NoVs by RT-PCR, and seven different genotypes were identified: GI-1, GI-4, GII-1, GII-3, GII-4, GII-6 and GII-13, of which GII-4 was the most prevalent (29/42, 69.0 %). Phylogenetic and Simplot analyses showed that three recombinant strains were detected: two GII-4 polymerase/GII-3 capsid recombinants and one GII-6 polymerase/GII-4 capsid recombinant. This study indicated that NoV was a common causative agent of sporadic acute gastroenteritis in adults in Ji’nan, China, and that NoV GII-4 was the predominant strain during this period. Three recombinant strains were identified in which GII-6 polymerase/GII-4 capsid was detected for the first time in China.

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Acknowledgments

This study was supported by grants from the Natural Science Foundation of Shandong Province (No. Y2008C68).

Conflict of interest

The authors declare that they had no conflict of interest.

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Authors and Affiliations

  1. Department of Infectious Diseases, Qilu Hospital, Shandong University, Wenhua Xi Road 107, Jinan, 250012, Shandong, China

    Lintao Sai, Gang Wang, Haihong Liu, Yajun Zhang, Chunmei Qu & Lixian Ma

  2. Department of Laboratory Sciences, School of Public Health, Shandong University, Wenhua Xi Road 44, Jinan, 250012, Shandong, China

    Lihua Shao

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  1. Lintao Sai
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  2. Gang Wang
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Correspondence to Lixian Ma.

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Sai, L., Wang, G., Shao, L. et al. Clinical and molecular epidemiology of norovirus infection in adults with acute gastroenteritis in Ji’nan, China. Arch Virol 158, 2315–2322 (2013). https://doi.org/10.1007/s00705-013-1753-0

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  • DOI: https://doi.org/10.1007/s00705-013-1753-0

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