Abstract
From the plant pathogen Ustilaginoidea virens, four double-stranded RNA (dsRNA) segments designated Uv-dsRNA1, -2, -3, and -4 were isolated, cloned, and sequenced. Uv-dsRNA1 (1775 bp) and -2 (1588 bp) potentially encode an RNA-dependent RNA polymerase (RdRp) and a viral coat protein (CP), respectively. Since the RdRp and CP sequences encoded by Uv-dsRNA1 and -2, respectively, are most closely related to, but clearly distinct from, those of viruses of the genus Partitivirus, they appear to be the two genome segments of a new partitivirus, for which the name Ustilaginoidea virens partitivirus 1 is proposed. In contrast, Uv-dsRNA3 (1352 bp) did not share significant sequence similarity with GenBank sequences, and the ORF of Uv-dsRNA4 (1119 bp) was only 32 % identical to a functionally unknown protein (GaRVMS2s3gp1) encoded by Gremmeniella abietina RNA virus MS2.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Ann Rev Phytopath 47:353–384
Ghabrial SA, Nibert ML, Maiss E, Lesker T, Baker TS, Tao YJ (2011) Family partitiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: ninth report of the international committee on taxonomy of viruses. Elsevier, USA, pp 523–534
Boccardo G, Lisa V, Luisoni E, Milne RG (1987) Cryptic plant viruses. Adv Virus Res 32:171–214
Ghabrial SA (1998) Origin, adaptation and evolutionary pathways of fungal viruses. Virus Genes 16(1):119–131
Khramtsov NV, Woods KM, Nesterenko MV, Dykstra CC, Upton SJ (1997) Virus-like, double-stranded RNAs in the parasitic protozoan Cryptosporidium parvum. Mol Microbiol 26:289–300
Oh CS, Hillman BI (1995) Genome organization of a partitivirus from the filamentous ascomycete Atkinsonella hypoxylon. J Gen Virol 76:1461–1470
Salem NM, Golino DA, Falk BW, Rowhani A (2008) Complete nucleotide sequences and genome characterization of a novel double-stranded RNA virus infecting Rosa multiflora. Arch Virol 153:455–462
Tuomivirta T, Hantula J (2005) Three unrelated viruses occur in a single isolate of Gremmeniella abietina var. abietina type A. Virus Res 110:31–39
Tzanetakis IE, Price R, Martin RR (2008) Nucleotide sequence of the tripartite Fragaria chiloensis cryptic virus and presence of the virus in the Americas. Virus Genes 36:267–272
Chiba S, Lin Y-H, Kondo H, Kanematsu S, Suzuki N (2013) Effects of defective interfering RNA on symptom induction by, and replication of, a novel partitivirus from a phytopathogenic fungus, Rosellinia necatrix. J Virol 87:2330–2341
Pearson M, Beever R, Boine B, Arthur K (2009) Mycoviruses of filamentous fungi and their relevance to plant pathology. Molecular Plant Pathol 10:115
Herrero N, Dueñas E, Moraga EQ, Zabalgogeazcoa I (2012) Prevalence and diversity of viruses in the entomopathogenic fungus Beauveria bassiana. Appl Environ Microbiol 78:8523–8530
Ghabrial SA, Suzuki N (2010) Fungal viruses. In: Van Mahy BWJ, Regenmortel MHV (eds) Desk encyclopedia of plant and fungal virology. Elsevier, Oxford, pp 517–524
Zhang T, Jiang Y, Huang J, Dong W (2013) Complete genome sequence of a putative novel victorivirus from Ustilaginoidea virens. Arch Virol. doi: 10.1007/s00705-013-1615-9
Froussard P (1993) rPCR: a powerful tool for random amplification of whole RNA sequences. Genome Res 2:185–190
Potgieter AC, Page NA, Liebenberg J, Wright IM, Landt O, van Dijk AA (2009) Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes. J Gen Virol 90:1423–1432
Kumar S, Nei M, Dudley J, Tamura K (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9(4):299–306
Buck KW (1996) Comparison of the replication of positive-stranded RNA viruses of plants and animals. Adv Virus Res 47:159–251
Rong R, Rao S, Scott SW, Carner GR, Tainter FH (2002) Complete sequence of the genome of two dsRNA viruses from Discula destructiva. Virus Res 90(1–2):217–224
Ghabrial SA, Ochoa WF, Baker TS, Nibert ML (2008) Partitiviruses: general features. In: Mahy BWJ, Van Regenmortel MHV (eds) Encyclopedia of virology, vol 5. Oxford, Elsevier, pp 68–75
Chen L, Chen J, Zhang H, Chen S (2006) Complete nucleotide sequences of three dsRNA segments from Raphanus sativus-root cv. Yipinghong with leaf yellow edge symptoms. Arch Virol 151:2077–2083
Acknowledgments
This work was supported by the National Major Project for Crop Breeding (2011ZX08003-001), the China Scholarship Council, and partially by the Special Fund for Agro-scientific Research in the Public Interest (200903039-3).
Author information
Authors and Affiliations
Corresponding author
Additional information
T. Zhang and Y. Jiang contributed equally to this work.
Electronic supplementary material
Below is the link to the electronic supplementary material.
705_2013_1742_MOESM1_ESM.tif
Supplementary Fig. 1 Agarose gel electrophoresis of dsRNAs extracted from asexual conidia spores of Ustilaginoidea virens strain JYH-ZT. The dsRNA samples that were not treated with RNase-free DNaseI were fractionated on 1.0% agarose gels and stained with ethidium bromide. Lane M, 1 Kb DNA Ladder Marker (TAKALA, Dalian, China); sizes in kb are indicated at the left. Lane 1-9, asexual spores containing dsRNA elements from U. virens are displayed. Lane 8 shows that UvRV1 was separated from Uv-dsRNA1, -2, -3, and -4. (TIFF 105 kb)
Rights and permissions
About this article
Cite this article
Zhang, T., Jiang, Y., Huang, J. et al. Genomic organization of a novel partitivirus from the phytopathogenic fungus Ustilaginoidea virens . Arch Virol 158, 2415–2419 (2013). https://doi.org/10.1007/s00705-013-1742-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-013-1742-3