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Detection and characterization of a divergent avian reovirus strain from a broiler chicken with central nervous system disease

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Abstract

Avian orthoreoviruses have been associated with a variety of diseases in chickens, including tenosynovitis, runting-stunting syndrome, hepatitis, myocarditis, osteoporosis, respiratory diseases, and central nervous system disease. The primary objective of our study was the molecular characterization of an avian reovirus strain, T1781, which was isolated from a broiler chicken with a central nervous system disorder in Hungary during 2012. The complete genome sequence was determined using a traditional sequencing method after cell culture adaptation of the strain. Sequence and phylogenetic analyses showed that T1781 shared only moderate nucleic acid sequence identity in several genes to previously analyzed reovirus strains from chickens, and each gene formed separate branches in the corresponding phylogenetic trees. The maximum nucleotide sequence identities of strain T1781 genes to reference avian reovirus strains ranged from 79 % to 90 %. Collectively, our analyses indicated that T1781 is a divergent chicken reovirus strain. The genetic background of this and other avian reoviruses associated with various disease manifestations needs further investigation.

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Acknowledgments

We are grateful to Jon R. Gentsch for reading the manuscript and giving valuable suggestions. Financial support was given by the Hungarian Academy of Sciences (Momentum program).

Conflict of interest

The authors declared that they had no conflicts of interest with respect to their authorship or the publication of this article.

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Authors and Affiliations

  1. Institute for Veterinary Medical Research, Centre of Agricultural Research, Hungarian Academy of Sciences, Budapest, P.O. Box 18, 1581, Hungary

    Eszter Dandár, Szilvia L. Farkas & Krisztián Bányai

  2. Veterinary Diagnostic Directorate, National Food Chain Safety Office, Budapest, Hungary

    Ádám Bálint & Katalin Szentpáli-Gavallér

  3. Veterinary Diagnostic Directorate, National Food Chain Safety Office, Debrecen, Hungary

    Sándor Kecskeméti

  4. Department of Medical Genetics, University of Pécs, Pécs, Hungary

    Péter Kisfali & Béla Melegh

  5. Szentágothai Research Center, University of Pécs, Pécs, Hungary

    Péter Kisfali & Béla Melegh

Authors
  1. Eszter Dandár
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  2. Ádám Bálint
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  3. Sándor Kecskeméti
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  4. Katalin Szentpáli-Gavallér
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  5. Péter Kisfali
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  6. Béla Melegh
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  7. Szilvia L. Farkas
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  8. Krisztián Bányai
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Correspondence to Krisztián Bányai.

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Dandár, E., Bálint, Á., Kecskeméti, S. et al. Detection and characterization of a divergent avian reovirus strain from a broiler chicken with central nervous system disease. Arch Virol 158, 2583–2588 (2013). https://doi.org/10.1007/s00705-013-1739-y

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  • DOI: https://doi.org/10.1007/s00705-013-1739-y

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