Abstract
The complete genome sequence of a monopartite begomovirus isolate infecting Creek Premna (Premna serratifolia L.) plants that exhibited leaf curl, vein swelling, and enation symptoms in Nha Trang, Vietnam, was cloned and sequenced. It comprises 2,753 nucleotides (JQ793786) and has a typical organization of begomoviruses DNA-A with AV1 and AV2 open reading frames (ORFs) in the viral-sense strand and AC1, AC2, AC3, AC4 and AC5 ORFs in the complementary-sense strand. The full-length genome sequence of the isolate (clone VN7) shared the highest level of nucleotide sequence identity (83 %) with the isolate IN:Pusa:Tb:10 of tobacco leaf curl Pusa virus (HQ180391). The phylogenetic relationship of VN7 to other begomoviruses was also investigated. VN7 grouped most closely with a clade containing begomoviruses from China, India and Japan. According to the current taxonomic criteria for the genus Begomovirus, family Geminiviridae, the isolate VN7 represents a new species, herein named “Premna leaf curl virus” (PrLCV).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Reference
Brown JK (2010) Bemisia: Phylogenetic biology of the Bemisia tabaci sibling species group. In: Stansly PA, Naranjo SE (eds) Bemisia: bionomics and management of a global pest. Dordrecht-Springer, Amsterdam, pp 31–67
Brown JK, Czosnek H (2002) Whitefly transmission of plant viruses. Adv Botanical Res 36:65–76
Brown JK, Fauquet CM, Briddon RW, Zerbini M, Moriones E, Navas-Castillo J (2012) Geminiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, ninth report of the ICTV. Elsevier/Academic Press, London, pp 351–373
Briddon RW, Markham PG (2000) Cotton leaf curl virus disease. Virus Res 71:151–159
Briddon RW, Mansoor S, Bedford ID, Pinner MS, Saunders K, Stanley J, Zafar Y, Malik KA, Markham PG (2001) Identification of DNA components required for induction of cotton leaf curl disease. Virology 285:234–243
Briddon RW (2003) Cotton leaf curl disease, a multicomponent begomovirus complex. Mol Plant Pathol 4:427–434
Cui XF, Tao XR, Xie Y, Fauquet CM, Zhou XP (2004) A DNAβ associated with tomato yellow leaf curl China virus is required for symptom induction. J Virol 78:13966–139744
Cui XF, Li YQ, Hu DW, Zhou XP (2005) Expression of a begomoviral DNA β gene in transgenic Nicotiana plants induced abnormal cell division. J Zhejiang Univ Sci B 6(2):83–86
Czosnek H, Laterrot H (1997) A worldwide survey of tomato yellow leaf curl viruses. Arch Virol 142:1391–1406
Doyle JJ (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou XP (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783–821
Jose J, Usha R (2003) Bhendi yellow vein mosaic disease in India is caused by association of a DNAβ satellite with a Begomovirus. Virology 305:310–317
Lazarowitz SG, Shepherd RJ (1992) Geminiviruses: genome structure and gene function. Crit Rev Plant Sci 11:327–349
Legg JP, Fauquet CM (2004) Cassava mosaic geminiviruses in Africa. Plant Mol Biol 56:585–599
Orozco BM, Hanley-Bowdoin L (1996) A DNA structure is required for Geminivirus replication origin function. J Virol 70:148–158
Rogers SG, Bisaro DM, Horsch RB, Fraley RT, Hoffmann NL, Brand L, Elmer JS, Lloyd AM (1986) Tomato golden mosaic virus a component DNA replicates autonomously in transgenic plants. Cell 45:593–600
Rojas MR, Gilbertson RL, Russell DR, Maxwell DP (1993) Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Dis 77:340–347
Saunders K, Bedford ID, Briddon RW, Markham PG, Wong SM, Stanley J (2000) A unique virus complex causes ageratum yellow vein disease. Proc Natl Acad Sci U S A 97:6890–6895
Saunders K, Norman A, Gucciardo S, Stanley J (2004) The DNA β satellite component associated with ageratum yellow vein disease encodes an essential pathogenicity protein (βC1). Virology 324:37–47
Saeed M, Behjatnia SAA, Mansoor S, Zafar Y, Hasnain S, Rezaian MA (2005) A single complementary-sense transcript of a geminiviral DNAβ satellite is determinant of pathogenicity. Mol Plant Microbe Interact 18:7–14
Zhou XP, Xie Y, Tao XR, Zhang ZK, Li ZH, Fauquet CM (2003) Characterization of DNAβ associated with begomoviruses in China and evidence for coevolution with their cognate viral DNA-A. J Gen Virol 84:237–247
Acknowledgments
This work was supported by the National Nonprofit Research Program of China (201003065), the International Science and Technology Collaboration Program of China (2011DFB30040), the Science and Technology Program of Guangdong, China (2011B031500021), and the International Science and Technology Collaboration Program of Guangdong, China (2011B050400003).
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
She, X., He, Z. & Brown, J.K. A new, previously undescribed monopartite begomovirus infecting Premna serratifolia in Vietnam. Arch Virol 158, 2425–2428 (2013). https://doi.org/10.1007/s00705-013-1729-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-013-1729-0