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Characterization of Rhynchosia yellow mosaic Yucatan virus, a new recombinant begomovirus associated with two fabaceous weeds in Yucatan, Mexico

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Abstract

Rhynchosia minima (L.) DC. (Fabaceae) plants exhibiting bright golden mosaic symptoms were previously associated with begomovirus infection in Yucatan, México [1]. To characterize the begomovirus infecting these plants, the complete bipartite genome was cloned and sequenced. Sequence comparisons indicated that the virus was distinct from all other begomoviruses known to date, including those previously identified from symptomatic R. minima, and the name Rhynchosia yellow mosaic Yucatan virus (RhYMYuV) is proposed. Pairwise comparisons indicated that RhYMYuV DNA-A [2,597 nt, (EU021216)] and DNA-B [2,542 nt, (FJ792608)] components shared the highest nt sequence identity with Cabbage leaf curl virus (CaLCuV), 87% for component A and 71% for component B. Phylogenetic analysis indicated that both components of RhYMYuV are most closely related to other New World begomoviruses, having as closest relatives immediate outliers to the major Squash leaf curl virus (SLCV) clade. Recombination analysis of the RhYMYuV genome indicated that the DNA-A component has arisen through intermolecular recombination. R. minima plants inoculated with the monomeric clones developed a bright yellow mosaic similar to symptoms observed in naturally infected plants, confirming that the clones were infectious. Nicotiana benthamiana plants biolistically inoculated with monomeric clones developed curling and chlorosis in the newly emerging leaves. RhYMYuV was also detected in symptomatic Desmodium sect. Scorpiurus Benth. (Fabaceae) that were collected near the RhYMYuV-infected plants.

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Acknowledgments

Cecilia Hernández-Zepeda acknowledges postdoctoral fellowship support from Consejo Nacional de Ciencia y Tecnología (Conacyt, México) and Cinvestav. This research was partially supported by a Conacyt grant to RFRB. We thank Salvador Ambriz-Granados for his technical assistance.

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Authors and Affiliations

  1. Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del IPN (Cinvestav)-Unidad Irapuato, Irapuato, GTO, Mexico

    C. Hernández-Zepeda & R. F. Rivera-Bustamante

  2. School of Plant Sciences, The University of Arizona, Tucson, AZ, 85721, USA

    J. K. Brown & A. M. Idris

  3. Centro de Investigación Científica de Yucatán A. C, Unidad de Bioquímica y Biología Molecular de Plantas, Mérida, Yucatán, Mexico

    O. A. Moreno-Valenzuela

  4. División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, A.C, San Luis Potosí, SLP, Mexico

    G. Argüello-Astorga

  5. Herbarium CICY, Unidad de Recursos Naturales, Centro de Investigación Científica de Yucatán A. C, Mérida, Yucatán, Mexico

    G. Carnevali

Authors
  1. C. Hernández-Zepeda
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  2. J. K. Brown
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  3. O. A. Moreno-Valenzuela
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  4. G. Argüello-Astorga
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  6. G. Carnevali
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  7. R. F. Rivera-Bustamante
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Correspondence to R. F. Rivera-Bustamante.

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Hernández-Zepeda, C., Brown, J.K., Moreno-Valenzuela, O.A. et al. Characterization of Rhynchosia yellow mosaic Yucatan virus, a new recombinant begomovirus associated with two fabaceous weeds in Yucatan, Mexico. Arch Virol 155, 1571–1579 (2010). https://doi.org/10.1007/s00705-010-0730-0

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  • DOI: https://doi.org/10.1007/s00705-010-0730-0

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