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Effects of cytokines on long control region transcriptional activity in high-risk cutaneous human papillomavirus types 5 and 8

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Abstract

Cytokines play an important role in the control of mucosal HPV transcription. However, there is little data available on cutaneous HPVs, which are associated with non-melanoma skin cancers. Here, we describe a cell-based assay exploiting HaCaT keratinocytes stably transfected with a reporter construct containing the long control region (LCR) regulatory sequence of gene transcription in HPV-5 and HPV-8. This novel assay has allowed the first systematic analysis of the effects of cytokines on HPV-5 and HPV-8 LCR activity and provides a valuable tool for the search for cutaneous HPV-gene expression inhibitors.

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Acknowledgments

This work was supported by Turin University research funds and by grants from MIUR (PRIN 2008).

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Authors and Affiliations

  1. Department of Clinical and Biological Sciences, S. Luigi Gonzaga Hospital, University of Turin, Regione Gonzole, 10, 10043, Orbassano, Turin, Italy

    Manuela Donalisio, Alice Poli, Andrea Civra & David Lembo

  2. Department of Public Health and Microbiology, University of Turin, 10126, Turin, Italy

    Santo Landolfo

Authors
  1. Manuela Donalisio
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  2. Alice Poli
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  3. Andrea Civra
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  4. Santo Landolfo
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  5. David Lembo
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Correspondence to David Lembo.

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Donalisio, M., Poli, A., Civra, A. et al. Effects of cytokines on long control region transcriptional activity in high-risk cutaneous human papillomavirus types 5 and 8. Arch Virol 155, 583–587 (2010). https://doi.org/10.1007/s00705-010-0615-2

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  • DOI: https://doi.org/10.1007/s00705-010-0615-2

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