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Bovine immunodeficiency virus: identification of a long terminal repeat sequence with enhanced promoter activity

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Abstract

We previously identified a new bovine immunodeficiency virus (BIV) trans-activator factor of transcription (Tat236) that was derived from a variant of BIV. Here, we report a new BIV long terminal repeat (LTR) sequence (LTRn) that was obtained by PCR from the DNA of cells infected with the BIV variant mentioned above. Sequence analysis indicated that the LTRn U3 region harbors three nucleic acid mutations at residue positions −194, −135 and −114 when compared to the original (wild-type) LTR sequence. Reporter gene assays indicated that LTRn promotes basal and Tat-mediated transactivation activity to levels significantly higher than those obtained with the wild-type LTR. Restoration experiments to the wild-type genotype indicated that both the −135 and −114 nucleic acid substitutions were responsible for the enhanced promoter activity of BIV LTRn.

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Acknowledgments

M. Cojocariu was supported by a graduate studentship from “La Fondation UQAM”. This work was supported by an operating Discovery grant from the National Sciences and Engineering Research Council of Canada to D. Archambault.

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Authors and Affiliations

  1. Department of Biological Sciences, Université du Québec à Montréal, P. O. Box 8888, Succursale Centre-Ville, Montréal, Québec, H3C 3P8, Canada

    M. Cojocariu, M.-C. St-Louis & D. Archambault

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  1. M. Cojocariu
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  2. M.-C. St-Louis
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  3. D. Archambault
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Correspondence to D. Archambault.

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Cojocariu, M., St-Louis, MC. & Archambault, D. Bovine immunodeficiency virus: identification of a long terminal repeat sequence with enhanced promoter activity. Arch Virol 154, 1163–1167 (2009). https://doi.org/10.1007/s00705-009-0411-z

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  • DOI: https://doi.org/10.1007/s00705-009-0411-z

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