Skip to main content
SpringerLink
Log in

Genetic analysis and pathogenicity of betanodavirus isolated from wild redspotted grouper Epinephelus akaara with clinical signs

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Diseased wild redspotted grouper Epinephelus akaara were collected from Seto Inland Sea, Ehime Prefecture, in August 2002. Fish showed erratic swimming behavior and inflation of the swim bladder. The fish brains were positive for nodavirus in both RT-PCR and nested PCR. The sequence of the nested PCR product (177 nt) was closely related to that of a known betanodavirus, redspotted grouper nervous necrosis virus. When juvenile sevenband grouper E. septemfasciatus were challenged intravitreously with virus, abnormal swimming behavior and high mortality were observed. This is the first report on viral nervous necrosis in a wild population of redspotted grouper with clinical signs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Ball LA, Hendry DA, Johnson JE, Rueckert RR, Scotti PD (2000) Family nodaviridae. In: Van Regenmortel MHV, Fauquet CM, Bishop DHL, Carstens EB, Estes MK, Lemon SM, Manillof J, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB (eds) Virus taxonomy. Seventh report of the international committee on taxonomy of viruses. Academic Press, New York, pp 747–755

    Google Scholar 

  2. Barker DE, MacKinnon AM, Boston L, Burt MDB, Cone DK, Speare DJ, Griffiths S, Cook M, Ritchie R, Olivier G (2002) First report of piscine nodavirus infecting wild winter flounder Pleuronectes americanus in Passamaquoddy Bay, New Brunswick, Canada. Dis Aquat Org 49:99–105

    CAS  Google Scholar 

  3. Breuil G, Pepin JFP, Boscher S, Thiery R (2002) Experimental vertical transmission of nodavirus from broodfish to eggs and larvae of the sea bass, Dicentrarchus labrax. J Fish Dis 25:697–702

    Google Scholar 

  4. Chi SC, Lo CF, Kou GK, Chang PS, Peng SE, Chen SN (1997) Mass mortalities associated with viral nervous necrosis (VNN) disease in two species of hatchery-reared grouper, Epinephelus fuscogutatus and Epinephelus akaara. J Fish Dis 20:185–193

    Google Scholar 

  5. Frerichs GN, Rodger HD, Peric Z (1996) Cell culture isolation of piscine neuropathy nodavirus from juvenile sea bass, Dicentrarchus labrax. J Gen Virol 72:2537–2539

    Google Scholar 

  6. Glazebrook JS, Heasman MP, de Beer SW (1990) Picorna-like viral particles associated with mass mortalitites in larval barramundi, Lates calcarifer. J Gen Virol 77:2067–2071

    Google Scholar 

  7. Gomez DK, Sato J, Mushiake K, Isshiki T, Okinaka Y, Nakai T (2004) PCR-based detection of betanodaviruses from cultured and wild marine fish with no clinical signs. J Fish Dis 27:603–608

    PubMed  CAS  Google Scholar 

  8. Iwamoto T, Nakai T, Mori K, Arimoto M, Furusawa I (2000) Cloning of the fish cell line SSN-1 for piscine nodaviruses. Dis Aquat Org 43:81–89

    PubMed  CAS  Google Scholar 

  9. Iwamoto T, Okinaka Y, Mise K, Mori K, Arimoto M, Okuno T, Nakai T (2004) Identification of host-specificity determinants in betanodaviruses by using reassortants between striped jack nervous necrosis virus and sevenband grouper nervous necrosis virus. J Virol 78(3):1256–1262

    PubMed  CAS  Google Scholar 

  10. Johansen R, Sommerset I, Torud B, Korsnes K, Hjortaas MJ, Nilsen F, Nerland AH, Dannevig BH (2004) Characterization of nodavirus and viral encephalopathy and retinopathy in farmed turbot, Scophthalmus maximus (L.). J Fish Dis 27:591–601

    PubMed  CAS  Google Scholar 

  11. Mori K, Nakai T, Nagahara M, Muroga K, Mekuchi T, Kanno T (1991) A viral disease in hatchery-reared larvae and juveniles of redspotted grouper. Fish Pathol 26:209–210

    Google Scholar 

  12. Mori K, Nakai T, Muroga K, Arimoto M, Mushiake K, Furusawa I (1992) Properties of a new virus belonging to Nodaviridae found in larval striped jack Pseudocaranx dentex with nervous necrosis. Virology 187:368–371

    PubMed  CAS  Google Scholar 

  13. Mori K, Mangyoku T, Iwamoto T, Arimoto M, Tanaka S, Nakai T (2003) Serological relationships among genotypic variants of betanodavirus. Dis Aquat Org 57:19–26

    CAS  Google Scholar 

  14. Moruga K (2001) Viral and bacterial diseases of marine fish and shellfish in Japanese hatcheries. Aquaculture 202:23–44

    Google Scholar 

  15. Munday BL, Kwang J, Moody N (2002) Betanodavirus infections of teleost fish: a review. J Fish Dis 25:127–142

    Google Scholar 

  16. Nishizawa T, Furuhashi M, Nagai T, Nakai T, Muroga K (1997) Genomic classification of fish nodaviruses by molecular phylogenetic analysis of the coat protein gene. Appl Environ Microbiol 63:1633–1636

    PubMed  CAS  Google Scholar 

  17. Reed JL, Muench H (1938) A simple method for estimating fifty percent end points. Am J Hyg 27:493–497

    Google Scholar 

  18. Tanaka S, Aoki H, Nakai T (1998) Pathogenicity of the nodavirus detected from diseased sevenband grouper Epinephelus septemfasciatus. Fish Pathol 33:31–36

    CAS  Google Scholar 

  19. Yoshikoshi K, Inoue K (1990) Viral nervous necrosis in hatchery-reared larvae and juveniles of Japanese parrotfish, Oplegnathus fasciatus. J Fish Dis 13:69–77

    Google Scholar 

Download references

Acknowledgments

This study was supported in part by a Grant-in-Aid Scientific Research (B) (16380132) from Ministry of Education, Culture, Sports, Science and Technology, Japan.

Author information

Authors and Affiliations

  1. Brain Korea 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, South Korea

    Dennis K. Gomez

  2. Ehime Prefectural Chuyo Fisheries Experimental Station, Ehime, 799-3125, Japan

    Satoru Matsuoka

  3. National Research Institute of Aquaculture, Fisheries Research Agency, Saiki, 879-2602, Japan

    Koh-ichiro Mori

  4. Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, South Korea

    Se Chang Park

  5. Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan

    Yasushi Okinaka & Toshihiro Nakai

Authors
  1. Dennis K. Gomez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Satoru Matsuoka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Koh-ichiro Mori
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yasushi Okinaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Se Chang Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Toshihiro Nakai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Se Chang Park or Toshihiro Nakai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gomez, D.K., Matsuoka, S., Mori, Ki. et al. Genetic analysis and pathogenicity of betanodavirus isolated from wild redspotted grouper Epinephelus akaara with clinical signs. Arch Virol 154, 343–346 (2009). https://doi.org/10.1007/s00705-008-0305-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-008-0305-5

Keywords

Search

Navigation