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Appearance of neural stem cells around the damaged area following traumatic brain injury in aged rats

  • Translational Neurosciences - Original Article
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Abstract

We have previously reported free radical production after traumatic brain injury (TBI), which induces neural stem cell (NSC) degeneration and death. However, the effects of aging on NSC proliferation around the damaged area following TBI have not been investigated. Therefore, in this study, we used 10-week (young group) and 24-month-old (aged group) rat TBI models to investigate the effects of aging on NSC proliferation around damaged tissue using immunohistochemical and ex vivo techniques. Young and aged rats received TBI. At 1, 3 and 7 days after TBI, immunohistochemical and lipid peroxidation studies were performed. Immunohistochemistry revealed that the number of nestin-positive cells around the damaged area after TBI in the aged group decreased significantly when compared with those in the young group (P < 0.01). However, the number of 8-hydroxy-2′-deoxyguanosine-, 4-hydroxy-2-nonenal- and single-stranded DNA (ssDNA)-positive cells and the level of peroxidation around the damaged area after TBI significantly increased in the aged group, compared with those in the young group (P < 0.01). Furthermore, almost all ssDNA-positive cells in young and aged groups co-localized with NeuN and nestin staining. Ex vivo studies revealed that neurospheres, which differentiated into neurons and glia in culture, could only be isolated from injured brain tissue in young and aged groups at 3 days after TBI. These results indicate that, although there were fewer NSCs that have the potential to differentiate into neurons and glia, these NSCs escaped free radical-induced degeneration around the damaged area after TBI in the aged rat brain.

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Acknowledgments

We thank Mari Yachi for technical assistance.

Conflict of interest

The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Pathology, Faculty of Medicine, Kinki University, 377-2, Ohno-higashi, Osakasayama, Osaka, 589-8511, Japan

    Tatsuki Itoh, Akihiko Ito & Takao Satou

  2. Department of Surgery, Faculty of Medicine, Kinki University, Osaka, Japan

    Motohiro Imano

  3. Faculty of Pharmaceutical Sciences, Kinki University, Osaka, Japan

    Shozo Nishida, Masahiro Tsubaki & Takashi Nakayama

  4. Life Science Research Institute, Kinki University, Osaka, Japan

    Nobuyuki Mizuguchi & Shigeaki Yamanaka

  5. Department of Biochemistry, Faculty of Medicine, Kinki University, Osaka, Japan

    Masaki Tabuchi & Hiroshi Munakata

  6. Department of Pathology, PL Hospital, Osaka, Japan

    Shigeo Hashimoto

  7. Division of Hospital Pathology, Faculty of Medicine, Hospital of Kinki University, Osaka, Japan

    Takao Satou

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  1. Tatsuki Itoh
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  2. Motohiro Imano
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Correspondence to Tatsuki Itoh.

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Itoh, T., Imano, M., Nishida, S. et al. Appearance of neural stem cells around the damaged area following traumatic brain injury in aged rats. J Neural Transm 120, 361–374 (2013). https://doi.org/10.1007/s00702-012-0895-7

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  • DOI: https://doi.org/10.1007/s00702-012-0895-7

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