Abstract
We compared protein expression by Western blot analysis in four areas of postmortem brain from patients with schizophrenia and control subjects for several proteins that are often used as controls for Western blot studies: β-tubulin, actin, glyceraldehyde-3-phosphate dehydrogenase, and valosin-containing protein. We did not detect any differences in expression between subjects with schizophrenia and a comparison group. These results suggest that all four proteins are suitable loading controls for postmortem studies of schizophrenia.
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References
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Eastwood SL, Harrison PJ (2001) Synaptic pathology in the anterior cingulate cortex in schizophrenia and mood disorders. A review and a Western blot study of synaptophysin, GAP-43 and the complexins. Brain Res Bull 55(5):569–578
Ferguson RE, Carroll HP, Harris A, Maher ER, Selby PJ, Banks RE (2005) Housekeeping proteins: a preliminary study illustrating some limitations as useful references in protein expression studies. Proteomics 5(2):566–571
Liu NK, Xu XM (2006) Beta-tubulin is a more suitable internal control than beta-actin in western blot analysis of spinal cord tissues after traumatic injury. J Neurotrauma 23(12):1794–1801
McNulty SE, Toscano WA Jr (1995) Transcriptional regulation of glyceraldehyde-3-phosphate dehydrogenase by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin. Biochem Biophys Res Commun 212(1):165–171
Perrot-Sinal TS, Davis AM, McCarthy MM (2001) Developmental sex differences in glutamic acid decarboxylase (GAD(65)) and the housekeeping gene, GAPDH. Brain Res 922(2):201–208
Stan AD, Ghose S, Gao XM, Roberts RC, Lewis-Amezcua K, Hatanpaa KJ, Tamminga CA (2006) Human postmortem tissue: what quality markers matter? Brain Res 1123(1):1–11
Wang Q, Song C, Li CC (2004) Molecular perspectives on p97-VCP: progress in understanding its structure and diverse biological functions. J Struct Biol 146(1–2):44–57
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Supported by MH53327 (JMW), MH78378 (DEB), MH064673 & MH066392 (VH) and MH074016 (REM).
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Bauer, D.E., Haroutunian, V., McCullumsmith, R.E. et al. Expression of four housekeeping proteins in elderly patients with schizophrenia. J Neural Transm 116, 487–491 (2009). https://doi.org/10.1007/s00702-008-0143-3
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DOI: https://doi.org/10.1007/s00702-008-0143-3