Abstract
For early diabetes identification and management, the progression of an uncomplicated and exceedingly responsive glucose testing technology is crucial. In this study, we present a new sensor incorporating a composite of metal organic framework (MOF) based on cobalt, coated with boronic acid to facilitate selective glucose binding. Additionally, we successfully employed a highly sensitive electro-optical immunosensor for the detection of subtle changes in concentration of the diabetes biomarker glycated haemoglobin (HbA1c), using zeolitic imidazolate framework-67 (ZIF-67) coated with polydopamine which further modified with boronic acid. Utilizing the polymerization characteristics of dopamine and the NH2 groups, a bonding structure is formed between ZIF-67 and 4-carboxyphenylboronic acid. ZIF-67 composite served as an effective substrate for immobilising 4-carboxyphenylboronic acid binding agent, ensuring precise and highly selective glucose identification. The sensing response was evaluated through both electrochemical and optical methods, confirming its efficacy. Under optimized experimental condition, the ZIF-67 based sensor demonstrated a broad detection range of 50–500 mg dL−1, a low limit of detection (LOD) of 9.87 mg dL−1 and a high correlation coefficient of 0.98. Furthermore, the 4-carboxyphenylboronic acid-conjugated ZIF-67-based sensor platform exhibited remarkable sensitivity and selectivity in optical-based detection for glycated haemoglobin within the clinical range of 4.7–11.3%, achieving a LOD of 3.7%. These findings highlight the potential of the 4-carboxyphenylboronic acid-conjugated ZIF-67-based electro-optical sensor as a highly sensitive platform for diabetes detection.
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Acknowledgements
The authors would like to thank the Director, CSIR-CSIO, Chandigarh, for providing necessary infrastructure and facilities. The authors also acknowledged the Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, for providing a dynamic and encouraging environment, which is appreciable.
Funding
Research reported in this paper was supported by Indian Council of Medical Research, India under senior research fellowship award from grant number 5/3/8/38/ITR-F/2020-ITR for project titled “Design and development of colorimetric technique-based point of care device for the detection of Glycated Haemoglobin.”
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Ethical approval was obtained from the Institutional Ethics Review Committee: CSIR-Central Scientific Instruments Organisation under Reference No. IEC/CSIO/2020/24, dated June 18, 2020, for the research project “Colorimetric sensing-based point of care device for the glycated haemoglobin.”
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Lakhera, P., Chaudhary, V., Kumar, P. et al. Nonenzymatic dual glucose sensing on boronic acid modified zeolitic imidazolate framework-67 nanoparticles for diabetes management. Microchim Acta 191, 306 (2024). https://doi.org/10.1007/s00604-024-06370-9
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DOI: https://doi.org/10.1007/s00604-024-06370-9