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One-pot wet-chemical fabrication of 3D urchin-like core-shell Au@PdCu nanocrystals for electrochemical breast cancer immunoassay

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Abstract

Carbohydrate antigen 15-3 (CA15-3) is an important biomarker for early diagnosis of breast cancer. Herein, a label-free electrochemical immunosensor was built based on three-dimensional (3D) urchin-like core-shell Au@PdCu nanocrystals (labeled Au@PdCu NCs) for highly sensitive detection of CA15-3, where K3[Fe(CN)6] behaved as an electroactive probe. The Au@PdCu NCs were synthesized by a simple one-pot wet-chemical approach and the morphology, structures, and electrocatalytic property were investigated by several techniques. The Au@PdCu NCs prepared worked as electrode material to anchor more antibodies and as signal magnification material by virtue of its exceptional catalytic property. The developed biosensor exhibited a wide linear detection range from 0.1 to 300 U mL−1 and a low limit of detection (0.011 U mL−1, S/N = 3) for determination of CA15-3 under the optimal conditions. The established biosensing platform exhibits some insights for detecting other tumor biomarkers in clinical assays and early diagnosis.

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Funding

This research was supported by the Zhejiang Public Welfare Technology Application Research Project (LGG19B050001).

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Authors and Affiliations

  1. Department of Breast Care Centre, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510080, China

    Di-Nan Chen, Jiu-Ju Feng & Tuck Yun Cheang

  2. College of Chemistry and Materials Science, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, China

    Di-Nan Chen & Ai-Jun Wang

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  1. Di-Nan Chen
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Correspondence to Jiu-Ju Feng or Tuck Yun Cheang.

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Chen, DN., Wang, AJ., Feng, JJ. et al. One-pot wet-chemical fabrication of 3D urchin-like core-shell Au@PdCu nanocrystals for electrochemical breast cancer immunoassay. Microchim Acta 190, 353 (2023). https://doi.org/10.1007/s00604-023-05932-7

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