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A biosensor based on Fe3O4@MXene-Au nanocomposites with high peroxidase-like activity for colorimetric and smartphone-based detection of glucose

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Abstract

A novel magnetic nanozyme Fe3O4@MXene-Au nanocomposite, which possessed higher peroxidase-like activity than that of Fe3O4 nanoparticles and Fe3O4@MXene nanocomposites, was developed. The outstanding magnetic properties of the nanozyme endowed it with the ability of simple and rapid separation, achieving great recyclability. Based on Fe3O4@MXene-Au nanocomposites and glucose oxidase (Glu Ox), a highly selective colorimetric biosensor for glucose detection was developed. Fe3O4@MXene-Au nanocomposites can catalyze H2O2 produced from glucose catalyzed by glucose oxidase to ·OH and oxidize colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB (oxTMB) with a significant absorbance at 652 nm. The linear range of glucose was 0–1.4 mM under optimal conditions, with a limit of detection (LOD) of 0.11 mM. Glucose in human whole blood was successfully detected with satisfactory recoveries. Furthermore, a facile agarose hydrogel detection platform was designed. With smartphone software, glucose detection can be realized by the agarose hydrogel platform, demonstrating the potential in on-site and visual detection of glucose.

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Funding

This work was supported by the National Key Research and Development Program of China (No. 2021YFF1200800, 2022YFA1104800), the National Natural Science Foundation of China (No. 81773681, 21904069, 22274079), the Natural Science Foundation of Jiangsu Province (No. BK20190653), Chinese Postdoctoral Science Foundation (No. 2017M621789), and Medical Research Foundation of Jiangsu Commission of Health (No. Z2020009).

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  1. Jianwen Fei and Wei Yang contributed equally to this work.

Authors and Affiliations

  1. School of Pharmacy, Nanjing Medical University, Nanjing, 211166, Jiangsu, China

    Jianwen Fei, Wei Yang, Yin Dai, Wei Xu, Huizhu Fan, Yani Zheng, Jun Zhang, Wanying Zhu, Junli Hong & Xuemin Zhou

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  1. Jianwen Fei
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Fei, J., Yang, W., Dai, Y. et al. A biosensor based on Fe3O4@MXene-Au nanocomposites with high peroxidase-like activity for colorimetric and smartphone-based detection of glucose. Microchim Acta 190, 336 (2023). https://doi.org/10.1007/s00604-023-05900-1

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