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Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite

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Abstract

An electrochemical impedimetric biosensor for human serum albumin (HSA) determination is proposed. The biosensor is based on water-phase assembled nanocomposites made of 2D WS2 nanoflakes and Au nanoparticles (AuNPs). The WS2 has been produced using a liquid-phase exfoliation strategy assisted by sodium cholate, obtaining a water-stable suspension that allowed the straightforward decoration with AuNPs directly in the aqueous phase. The resulting WS2/Au nanocomposite has been characterized by atomic force microscopy and Raman spectroscopy and, then, employed to modify screen-printed electrodes. Good electron-transfer features have been achieved. An electrochemical immunosensing platform has been assembled exploiting cysteamine-glutaraldehyde covalent chemistry for antibody (Ab) immobilization. The resulting immunosensor exhibited good sensitivity for HSA detection (LOD = 2 ng mL−1), with extended linear range (0.005 – 100 µg mL−1), providing a useful analytical tool for HSA determination in urine at relevant clinical ranges for microalbuminuria screening. The HSA quantification in human urine samples resulted in recoveries from 91.8 to 112.4% and was also reproducible (RSD < 7.5%, n = 3), with marked selectivity. This nanocomposite, thanks to the reliable performance and the ease of the assembling strategy, is a promising alternative for electrochemical immunosensing of health relevant markers.

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Acknowledgements

We acknowledge CF Nanobiotechnology and CF Cryo-electron microscopy and tomography of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2023042).

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Authors and Affiliations

  1. Department of Bioscience and Technology for Food, Agriculture and Environment, Campus “Aurelio Saliceti”, Via R Balzarini 1, 64100, Teramo, Italy

    Filippo Silveri & Dario Compagnone

  2. Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic

    Filippo Silveri, Radka Obořilová, Jakub Máčala & Petr Skládal

  3. CEITEC MU—Nanobiotechnology, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic

    Radka Obořilová & Petr Skládal

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  1. Filippo Silveri
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Silveri, F., Obořilová, R., Máčala, J. et al. Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite. Microchim Acta 190, 306 (2023). https://doi.org/10.1007/s00604-023-05873-1

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