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Diagnostic genosensor for detection of rotavirus based on HFGNs/MXene/PPY signal amplification

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Abstract

A novel genosensor was developed for rotavirus specific cDNA sequence detection. The genosensor was comprised of hierarchical flower-like gold nanostructures, MXene, and polypyrrole (HFGNs/MXene/PPY) nanocomposite as a signal amplification tag, specific antisense ssDNA oligonucleotide as a recognition bioelement, and methylene blue (MB) as a redox marker. The morphological and electrochemical features of the biosensor were first tested and optimized and the high performance of the platform was confirmed in terms of sensitivity and reproducibility. Then, 20 rotavirus RNA isolated from clinical and cell-cultured samples (10 positive and 10 negative confirmed by RT-PCR and electrophoresis methods) were evaluated by the genosensor. The analysis results revealed that the genosensor is able to differentiate successfully between the positive and negative control groups. The developed genosensor for rotavirus RNA detection presented an excellent limit of detection of ∼ 0.8 aM and a determination  range of  10−18 and 10−7 M. In addition, the ssDNA/HFGNs/MXene/PPY/GCE showed high selectivity and long-term stability of ~ 24 days. Therefore, this novel genosensor would be of great benefit for the clinical diagnosis of rotavirus.

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Funding

This study was financially supported by (1) Faculty of Medical Sciences, Tarbiat Modares University of Tehran (Grant No. 86258), (2) The Deputy of Research, Mashhad University of Medical Sciences (Grant No. 4002031), Mashhad, Iran.

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Authors and Affiliations

  1. Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, 1411713116, Iran

    Kiana Ketabi & Hoorieh Soleimanjahi

  2. Department of Virology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, 6517838736, Iran

    Ali Teimoori

  3. Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, 1313191379, Iran

    Behnaz Hatamluyi & Zahra Meshkat

  4. Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, 1313191379, Iran

    Majid Rezayi

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  1. Kiana Ketabi
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  2. Hoorieh Soleimanjahi
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  3. Ali Teimoori
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  4. Behnaz Hatamluyi
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  5. Majid Rezayi
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  6. Zahra Meshkat
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Correspondence to Hoorieh Soleimanjahi or Zahra Meshkat.

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Ketabi, K., Soleimanjahi, H., Teimoori, A. et al. Diagnostic genosensor for detection of rotavirus based on HFGNs/MXene/PPY signal amplification. Microchim Acta 190, 293 (2023). https://doi.org/10.1007/s00604-023-05871-3

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  • DOI: https://doi.org/10.1007/s00604-023-05871-3

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