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Surface plasmon resonance biosensor using hydrogel-AuNP supramolecular spheres for determination of prostate cancer-derived exosomes

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Abstract

Based on the hydrogel-AuNP supramolecular sphere (H-Au), a label-free and real-time surface plasmon resonance imaging biosensor has been developed for highly sensitive and specific determination of prostate cancer cell-derived exosomes. After integrating the signal amplification effect of the mass cumulative hydrogel and the LSPR effect of AuNPs with high specific aptamer, the SPRi biosensor for exosome detection exhibited a wide linear range from 1.00 × 105 to 1.00 × 107 particles/mL with a limit of detection of 1.00 × 105 particles/mL. Most importantly, with a strong correlation between the SPRi signal and the t-PSA value measured by the clinical chemiluminescence immunoassay, this biosensor displayed excellent practicability for human serum analysis, which exhibits great potential applications in disease diagnosis and bioanalysis.

Graphical abstract

Prostate cancer has been one of the most threatening diseases in human life and health nowadays. In particular, as cancer metastasizes, it is more likely to cause fracture, paraplegia, and even fatal consequences. However, the predominant t-PSA test needs further improvement for the deficiencies of limited specificity and sensitivity, which is prone to false positive. As one of the noninvasive markers of liquid biopsies, exosome has the potential to be a substitute for t-PSA, which can provide specific and predictive information in disease diagnosis and prognosis. Herein, based on the hydrogel-AuNP supramolecular sphere (H-Au), a label-free and real-time surface plasmon resonance biosensor has been developed for highly sensitive and specific detection of prostate cancer cell-derived exosomes. After integrating the signal amplification effect of mass cumulative hydrogel and LSPR effect of AuNPs with high specific aptamer, this developed SPRi biosensor for exosome detection exhibited a wide linear range from 1.00 × 105 to 1.00 × 107 particles/mL with a limit of detection down to 1.00 × 105 particles/mL. Most importantly, with a strong correlation between the SPRi signal and the t-PSA value measured by the clinical chemiluminescence immunoassay, this biosensor displayed excellent practicability in human serum, which exhibited great potential applications in disease diagnosis and bioanalysis.

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Funding

This work was supported by the National Natural Science Foundation of China (81873980, 81873972), the financial support from the National Science and Technology Major Project of the Ministry of Science and Technology of China (2018ZX10732202), and the Chongqing Medical University Graduate Talent Training Program (BJRC201908).

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Author notes

  1. Wenqin Chen and Jia Li contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China

    Wenqin Chen, Jia Li, Xiaotong Wei, Yunpeng Fan, Husun Qian & Shijia Ding

  2. Chongqing Engineering Research Center for Criminal Investigation Technology, Chongqing Medical University, Chongqing, 400016, China

    Siqiao Li & Shijia Ding

  3. The Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 40016, China

    Yu Xiang

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  1. Wenqin Chen
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Correspondence to Shijia Ding.

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Chen, W., Li, J., Wei, X. et al. Surface plasmon resonance biosensor using hydrogel-AuNP supramolecular spheres for determination of prostate cancer-derived exosomes. Microchim Acta 187, 590 (2020). https://doi.org/10.1007/s00604-020-04573-4

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