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Determination of 17β-estradiol by surface-enhanced Raman spectroscopy merged with hybridization chain reaction amplification on Au@Ag core-shell nanoparticles

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Abstract

The authors describe an aptamer-based assay for 17β-estradiol. It relies on the combined use of surface enhanced Raman scattering (SERS) and hybridization chain reaction (HCR). The aptamer against 17β-estradiol is applied as the recognition probes, and this results in excellent specificity. Specific recognition of target 17β-estradiol induce the freedom of DNA 2, which will open the stem-loop structure of probe 1 on the Au@Ag and form the partial dsDNA structure. With the nicking enzyme, the partial dsDNA will be hydrolyzed and the reside ssDNA on Au@Ag will form a small stem-loop structure. With the help of the other probe 2 modified Au@Ag and pre-immobilized probe 3 on the well of the microplate, an enzyme-free HCR can occur and tremendous Au@Ag can be assembled along the formed dsDNA in HCR, which can act as the excellent substrate for Raman measurement and greatly amplify the Raman signal of R6G on the Au@Ag. Afterwards, the key factor, ratio between probe 2-Au@Ag (P2) and probe1-Au@Ag (P1), affects the detection sensitivity is systematically optimized for the best sensing performance. The SERS signal of R6G, best measured at 1651 cm−1, increases linearly in the wide range from 1 pM to 10 nM. The detection limit can be as low as 0.1 pM.

Schematic presentation of an aptamer-based surface enhanced Raman scattering method for accurate detection of 17β-estradiol, which is integrated with hybridization chain reaction for signal amplification and sensitivity improvement.

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Acknowledgements

This work is financially supported by the grant of 2017YFF0208600, the NSFC Grant of 21475030, Open Foundation of Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization of EWPL201701, the China Agriculture Research System-48 (CARS-48), Anhui Provincial Modern Argo-industry Tech. Research System (NYCYTX-2016-84) and the Fundamental Research Fund for central university (Grants No. 2017HGPA0162, JZ2018HGTA0205, PA2017GDQT0018).

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  1. Li Yao and Yulin Li contributed equally to this manuscript.

Authors and Affiliations

  1. School of Food Science and Biological Engineering, Engineering Research Center of Bio-process, MOE, Hefei University of Technology, Hefei, 230009, China

    Li Yao, Jianguo Xu & Wei Chen

  2. Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Hubei Normal University, Huangshi, 435002, China

    Yulin Li

  3. Faculty of Marine Science, Ningbo University, Ningbo, 315211, China

    Kewen Cheng & Daodong Pan

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  1. Li Yao
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  2. Yulin Li
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  4. Daodong Pan
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  5. Jianguo Xu
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Correspondence to Jianguo Xu or Wei Chen.

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Yao, L., Li, Y., Cheng, K. et al. Determination of 17β-estradiol by surface-enhanced Raman spectroscopy merged with hybridization chain reaction amplification on Au@Ag core-shell nanoparticles. Microchim Acta 186, 52 (2019). https://doi.org/10.1007/s00604-018-3114-x

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