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Amplified photoelectrochemical immunoassay for the tumor marker carbohydrate antigen 724 based on dye sensitization of the semiconductor composite C3N4-MoS2

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Abstract

The authors describe an amplified photoelectrochemical immunoassay for the tumor marker carbohydrate antigen 724 (CA724). The method employs a C3N4-MoS2 semiconductor as the photoelectric conversion layer. The nanocomposite was characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray powder diffraction, and UV-vis diffuse reflectometry. The dye eosin Y was encapsulated into CaCO3 nanospheres which then were used as labels for antibody against CA724. In addition, Fe3O4 nanospheres were employed as magnetic platform for constructing photoelectrochemical sandwich immunoassay. The CaCO3 nanospheres can be dissolved with aid of ethylene diamine tetraacetic acid (EDTA) and the carried eosin Y in CaCO3 is released. The released dyes sensitizes the C3N4-MoS2 semiconductor, which induces photocurrent amplification. Under optimal conditions and at a typical working voltage of 0 V (vs. SCE), the photocurrent increases linearly in the range of 0.05 mU mL−1 to 500 mU mL−1 of CA724, with a 0.02 mU mL−1 detection limit.

The C3N4-MoS2 complex, with high efficiency of electron transport, was synthesized to construct a photoelectrochemical analytical platform. A sandwich-type immunoassay was established on the surface of magnetic beads. Carbohydrate antigen 724 in sample was detected sensitively by using sensitization of released eosin Y as signal amplifiery.

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Acknowledgements

The National Natural Science Foundation of China (Grant No. 21605111) and Natural Science Foundation of Shanxi Province (No. 201601D021037 and 201601D011079) are gratefully acknowledged.

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China

    Chuanmin Ding, Kaijing Song, Hongyun Meng, Bing Zhang, Zhihuan Zhao, Honghong Chang & Wenlong Wei

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  1. Chuanmin Ding
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  2. Kaijing Song
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  3. Hongyun Meng
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  4. Bing Zhang
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Correspondence to Bing Zhang.

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Ding, C., Song, K., Meng, H. et al. Amplified photoelectrochemical immunoassay for the tumor marker carbohydrate antigen 724 based on dye sensitization of the semiconductor composite C3N4-MoS2. Microchim Acta 185, 530 (2018). https://doi.org/10.1007/s00604-018-3054-5

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