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Voltammetric determination of ascorbic acid by using a disposable screen printed electrode modified with Cu(OH)2 nanorods

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Abstract

The authors describe a disposable non-enzymatic sensor for ascorbic acid (AA) that was obtained by modifying a screen printed electrode (SPE) with Cu(OH)2 nanorods (NRs). The NRs were synthesized by a wet chemical process which involves sequential addition of NH3 and NaOH to CuSO4 solution. NR formation was confirmed by thermogravimetric, spectroscopic, microscopic, and diffraction studies. The Cu(OH)2 NRs were mixed with carbon ink and printed onto an SPE. Electrochemical detection of AA was carried out at pH 7.4, at a typical voltage as low as 0 mV versus saturated calomel electrode with a scan rate of 100 mV/s, and is assumed to involve the chemical reduction of Cu(II) by AA followed by electrochemical oxidation of Cu(I). The sensor has a linear response in the 0.0125 to 10 mΜ AA concentration range. Response to AA is free from interference by urea, glucose, uric acid, dopamine, metal ions such as Fe2+, Zn2+ and Ni2+, NaCl, KCl and ethanol. It was applied to the determination of AA in a vitamin C tablet and in urine.

A disposable non-enzymatic sensor for ascorbic acid was fabricated using Cu(OH)2 nanorods. The assay is based on the chemical reduction of Cu(II) by AA followed by an electrochemical oxidation of Cu(I). The sensor electrode showed excellent linearity (0.0125–10 mM), sensitivity (268 μA/mM/cm2) and selectivity.

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Acknowledgements

Authors greatly acknowledge the Department of Biotechnology (DBT), Government of India for the financial support under the project (Project No BT/PR4076/MED/32/221/2011) and services rendered by PSGTechs COE Indutech, PSG College of Technology, Neelambur. Jeethu Raveendran express her sincere thanks to Council of Scientific and Industrial Research (CSIR) for the financial support under CSIR-SRF Direct scheme (Sanction No. 09/1034(0003)/2 K15-EMR-I).

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Authors and Affiliations

  1. Department of Sciences, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Amrita University, Coimbatore, 641112, India

    Jeethu Raveendran, Rajasree G. Krishnan & T. G. Satheesh Babu

  2. Department of ECE, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Amrita University, Coimbatore, 641112, India

    Jeethu Raveendran

  3. Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amrita University, Amritapuri, 641112, India

    Bipin G. Nair

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  1. Jeethu Raveendran
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  2. Rajasree G. Krishnan
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  3. Bipin G. Nair
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  4. T. G. Satheesh Babu
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Correspondence to T. G. Satheesh Babu.

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Raveendran, J., Krishnan, R.G., Nair, B.G. et al. Voltammetric determination of ascorbic acid by using a disposable screen printed electrode modified with Cu(OH)2 nanorods. Microchim Acta 184, 3573–3579 (2017). https://doi.org/10.1007/s00604-017-2391-0

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  • DOI: https://doi.org/10.1007/s00604-017-2391-0

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