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Voltammetric sensor for trichloroacetic acid using a glassy carbon electrode modified with Au@Ag nanorods and hemoglobin

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Abstract

Core-shell Au@Ag nanorods (Ag@GNRs) were synthesized and utilized to construct a voltammetric biosensor for trichloroacetic acid (TCA). The biosensor was prepared by immobilizing hemoglobin (Hb) on a glassy carbon electrode (GCE) that was modified with the Ag@GNRs. Cyclic voltammetry revealed a pair of symmetric redox peaks, indicating that direct electron transfer occurs at the Hb on the Ag@GNR-film. The electron transfer rate constant is as high as 2.32 s−1. The good electrocatalytic capability and large surface area of the Ag@GNR-film is beneficial in terms of electron transfer between Hb and the underlying electrode. The modified GCE, best operated at −0.4 V (vs. SCE), exhibits electrocatalytic activity toward TCA in the 0.16 μM to 1.7 μM concentration range, with a 0.12 μM detection limit (at an S/N ratio of 3).

Core-shell Au@Ag nanorods (Ag@GNRs) were synthesized and used to immobilize hemoglobin to construct an effective biosensor for trichloroacetic acid.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (21375066 and 21475070), the Natural Science Foundation of Jiangsu Province (BK20151267), the Qing Lan Project of Jiangsu Province, the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and the Application Research Item of Nantong City (MS12015046).

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  1. School of Public Health, Nantong University, Nantong, 226019, People’s Republic of China

    Dongping Qian, Weibo Li, Fangting Chen, Ying Huang, Ning Bao, Haiying Gu & Chunmei Yu

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  1. Dongping Qian
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  2. Weibo Li
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  3. Fangting Chen
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Correspondence to Haiying Gu or Chunmei Yu.

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Qian, D., Li, W., Chen, F. et al. Voltammetric sensor for trichloroacetic acid using a glassy carbon electrode modified with Au@Ag nanorods and hemoglobin. Microchim Acta 184, 1977–1985 (2017). https://doi.org/10.1007/s00604-017-2175-6

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