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Cellular biosensor based on red blood cells immobilized on Fe3O4 Core/Au Shell nanoparticles for hydrogen peroxide electroanalysis

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Abstract

A core/shell Fe3O4/gold nanocomposite was prepared for immobilizing of red blood cells on a gold electrode via conjugation to a cysteamine monolayer. The hemoglobin in the film undergoes direct electron transfer at a formal potential of −330 mV and displays excellent electrocatalytic response to hydrogen peroxide, with a linear range from 9.6 µM to 2.6 mM. The limit of detection is 4.4 µM (S/N = 3). The Michaelis–Menten constant is 120 µM. Owing to its good biocompatibility, the biosensor exhibits good stability and acceptable reproducibility. The nanocomposite film provided a good matrix for the immobilization of cells and for the preparation of cellular biosensors.

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Authors and Affiliations

  1. Institute of Analytical Chemistry for Life Science, School of Public Health, Nantong University, Nantong, 226007, People’s Republic of China

    Chao Chen, Yang Liu & Hai-Ying Gu

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  1. Chao Chen
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  2. Yang Liu
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  3. Hai-Ying Gu
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Correspondence to Hai-Ying Gu.

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Chen, C., Liu, Y. & Gu, HY. Cellular biosensor based on red blood cells immobilized on Fe3O4 Core/Au Shell nanoparticles for hydrogen peroxide electroanalysis. Microchim Acta 171, 371–376 (2010). https://doi.org/10.1007/s00604-010-0367-4

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  • DOI: https://doi.org/10.1007/s00604-010-0367-4

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