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Development of micro-flow devices by direct-milling on poly(methyl methacrylate) substrates with integrated optical detection

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Abstract

The development of micro-flow devices by direct-milling on poly(methyl methacrylate) (PMMA) substrates and incorporating the optic detector into the same structure is presented, evaluated and applied to the determination of chlorhexidine. Hydrodynamic characteristics of the developed devices were compared against those obtained from flow systems based on classic Teflon reactors. The optical detector integrated in the PMMA substrate is composed of a light emitting diode (λ = 627 nm) and light dependence resistance as light source and light detector, respectively. The performance of the optical detector was evaluated and optimized. The applicability of the fluidic device was assessed in the determination of chlorhexidine through its colorimetric reaction with bromocresol green. Micro-pumps were used as propulsion units. Results obtained in real sample analysis were in good agreement with those obtained by the reference procedure.

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Acknowledgements

One of us (E. R. G. O. Rodrigues) acknowledges Fundação para a Ciência e a Tecnologia (FCT) for the PhD grant (SFRH/BD/23417/2005).

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Authors and Affiliations

  1. REQUIMTE, Serviço de Química-Física, Faculdade de Farmácia da Universidade do Porto, Rua Aníbal Cunha 164, 4099-030, Porto, Portugal

    Eunice R. G. O. Rodrigues & Rui A. S. Lapa

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  1. Eunice R. G. O. Rodrigues
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  2. Rui A. S. Lapa
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Correspondence to Rui A. S. Lapa.

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Rodrigues, E.R.G.O., Lapa, R.A.S. Development of micro-flow devices by direct-milling on poly(methyl methacrylate) substrates with integrated optical detection. Microchim Acta 166, 189–195 (2009). https://doi.org/10.1007/s00604-009-0207-6

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  • DOI: https://doi.org/10.1007/s00604-009-0207-6

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