Abstract.
A sensitive chemiluminescence method for the determination of clindamycin is presented. The method is based on the inhibitory effect of clindamycin on the chemiluminescence reaction between luminol and myoglobin in a flow-injection system. The decrement in chemiluminescence intensity is linear with the logarithm of the clindamycin concentration over the range of 0.1–70.0 ng mL−1 (r 2 = 0.9995), with a detection limit of 0.03 ng mL−1 (3σ). At a flow rate of 2.0 mL min−1, the complete analytical process could be performed within 0.5 min, including sampling and washing, with a relative standard deviation of less than 3.0% (n = 5). The procedure was applied to the determination of clindamycin in human serum and in monitoring the excretion of clindamycin in human urine samples without any pretreatment process. It was found that the excretive clindamycin concentration reached its maximum 3 hours after oral administration. The clindamycin excretive ratio in 9 hours was 10.84% in the body of the volunteer.
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References
H Fieger-Büschges G Schlüssler V Larsimont H Blume (1999) ArticleTitleDetermination of clindamycin in human plasma by high-performance liquid chromatography using coupled columns J Chromatogr B 724 281
J F Prescott J D Baggot R D Walker (2000) Antimicrobial therapy in veterinary medicine EditionNumber3 Iowa State University Press Iowa
A S Amin (1995) ArticleTitleSpectrophotometric and conductometric determination of clindamycin hydrochloride in pure form and in pharmaceutical preparations Analusis 23 415 Occurrence Handle1:CAS:528:DyaK28XhtFOju7s%3D
F A El-Yazbi S M Blaih (1993) ArticleTitleSpectrophotometric and titrimetric determination of clindamycin hydrochloride in pharmaceutical preparations Analyst 118 577 Occurrence Handle10.1039/an9931800577 Occurrence Handle1:CAS:528:DyaK3sXktFGnsbg%3D
H Wan A G Holmen Y D Wang (2003) ArticleTitleHigh-throughput screening of pKa values of pharmaceuticals by pressure-assisted capillary electrophoresis and mass spectrometry Rapid Commun Mass Sp 17 2639 Occurrence Handle10.1002/rcm.1229 Occurrence Handle1:CAS:528:DC%2BD3sXpslSiurs%3D
P R Tiller L A Romanyshyn U D Neue (2003) ArticleTitleFast LC/MS in the analysis of small molecules Anal Bioanal Chem 377 788 Occurrence Handle10.1007/s00216-003-2146-0 Occurrence Handle1:CAS:528:DC%2BD3sXot1ersLY%3D
S Croubels S De Baere P De Backer (2003) ArticleTitlePractical approach for the stability testing of veterinary drugs in solutions and in biological matrices during storage Anal Chim Acta 483 419 Occurrence Handle10.1016/S0003-2670(02)01479-4 Occurrence Handle1:CAS:528:DC%2BD3sXjtFenu7Y%3D
R N Rao V Nagaraju (2003) ArticleTitleAn overview of the recent trends in development of HPLC methods for determination of impurities in drugs J Pharm Biomed Anal 33 335 Occurrence Handle10.1016/S0731-7085(03)00293-0
G C Batzias G A Delis P M Koutsoviti (2004) ArticleTitleA new HPLC/UV method for the determination of clindamycin in dog blood serum J Pharm Biomed Anal 35 545 Occurrence Handle10.1016/j.jpba.2004.01.022 Occurrence Handle1:CAS:528:DC%2BD2cXjvVCjtL0%3D
Y R Ye E Bektic R Buchta (2004) ArticleTitleSimultaneous determination of tretinoin and clindamycin phosphate and their degradation products in topical formulations by reverse phase HPLC J Sep Sci 27 71 Occurrence Handle10.1002/jssc.200301652 Occurrence Handle1:CAS:528:DC%2BD2cXpvVyksA%3D%3D
M A Targove N D Danielson (1990) ArticleTitleHigh-performance liquid chromatography of clindamycin antibiotics using tris(bipyridine)ruthenium(III) chemiluminescence detection J Chromatogr Sci 28 505 Occurrence Handle1:CAS:528:DyaK3MXht1aktA%3D%3D
A Economou D G Themelis G Theodoridis P D Tzanavaras (2002) ArticleTitleSensitive determination of captopril by flow injection analysis with chemiluminescence detection based on the enhancement of the luminol reaction Anal Chim Acta 463 249 Occurrence Handle10.1016/S0003-2670(02)00424-5 Occurrence Handle1:CAS:528:DC%2BD38XkvFGksb0%3D
A M Powe K A Fletcher (2004) ArticleTitleMolecular fluorescence, phosphorescence, and chemiluminescence spectrometry Anal Chem 76 4614 Occurrence Handle10.1021/ac040095d Occurrence Handle1:CAS:528:DC%2BD2cXkvFGkt7o%3D
A R Massimo (2003) ArticleTitleBioluminescence and chemiluminescence in drug screening Anal Bioanal Chem 377 826 Occurrence Handle10.1007/s00216-003-2096-6
L J Kricka (2003) ArticleTitleClinical applications of chemiluminescence Anal Chimica Acta 500 279 Occurrence Handle10.1016/S0003-2670(03)00809-2 Occurrence Handle1:CAS:528:DC%2BD3sXpt1Klu7o%3D
Z H Song N Zhang L Wang (2003) ArticleTitleIn vitro determination of dobesilate in medicine and human urine with chemiluminescence detection Microchim Acta 142 205 Occurrence Handle10.1007/s00604-003-0015-3 Occurrence Handle1:CAS:528:DC%2BD3sXmtF2htLs%3D
Z H Song C N Wang (2005) ArticleTitleDetermination of picogram-levels of acetylspiramycin in human urine and serum by flow injection chemiluminescence Microchim Acta 149 117 Occurrence Handle10.1007/s00604-004-0289-0 Occurrence Handle1:CAS:528:DC%2BD2MXksFynug%3D%3D
Z H Song S Hou (2003) ArticleTitleSub-picogram determination of vitamin B12 in pharmaceuticals and human serum using flow injection with chemiluminescence detection Anal Chimica Acta 488 71 Occurrence Handle10.1016/S0003-2670(03)00665-2 Occurrence Handle1:CAS:528:DC%2BD3sXlt1Wnsbg%3D
Z H Song L Wang S Hou (2004) ArticleTitleA study of the chemiluminescence behavior of myoglobin with luminol and its analytical applications Anal Bioanal Chem 378 529 Occurrence Handle10.1007/s00216-003-2302-6 Occurrence Handle1:CAS:528:DC%2BD2cXjtVWrtA%3D%3D
S K Huang (1989) Comprehensive textbook of pharmacokinetics Shanghai Medical University Press Shanghai
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Shao, X., Xie, X. & Song, Z. In vitro monitoring of clindamycin in human urine using flow injection chemiluminescence. Microchim Acta 157, 159–164 (2007). https://doi.org/10.1007/s00604-006-0666-y
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DOI: https://doi.org/10.1007/s00604-006-0666-y