Skip to main content
SpringerLink
Log in

Multi-syringe flow injection system for the determination of the scavenging capacity of the diphenylpicrylhydrazyl radical in methanol and ethanolic media

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract.

A multi-syringe flow injection system for the determination of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) scavenging capacity using methanol or ethanolic solutions as reaction media is presented. A stopped flow approach was implemented in order to monitor the DPPH• consumption using spectrophotometry at 517 nm. The kinetic profile for the first 3 min of reaction was determined for several antioxidant compounds and the number of DPPH• molecules reduced by one molecule of antioxidant was calculated whenever the absorbance value was stable within the period of measurement. For ascorbic acid, trolox, isoeugenol and quercetin in methanol, the values obtained were similar to those reported in the literature. It was possible to perform 14 determinations per hour, consuming 0.34 µmol of DPPH• per determination, which accounts for the application of this system as a screening tool for fast scavenger compounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • B Halliwell J M C Gutteridge (1999) Free radicals in biology and medicine EditionNumber2 Oxford University Press Oxford

    Google Scholar 

  • O I Aruoma (1994) Food Chem Toxicol 32 671 Occurrence Handle10.1016/0278-6915(94)90011-6 Occurrence Handle1:CAS:528:DyaK2cXltlaktbk%3D

    Article  CAS  Google Scholar 

  • M Antolovich P D Prenzler E Patsalides S McDonald K Robards (2002) Analyst 127 183 Occurrence Handle10.1039/b009171p Occurrence Handle1:CAS:528:DC%2BD38XhsFSntL0%3D

    Article  CAS  Google Scholar 

  • V Cerdà J M Estela R Forteza A Cladera E Becerra P Altimira P Sitjar (1999) Talanta 50 695 Occurrence Handle10.1016/S0039-9140(99)00196-4

    Article  Google Scholar 

  • M Miró V Cerdà J M Estela (2002) Trac Trends Anal Chem 21 199 Occurrence Handle10.1016/S0165-9936(02)00307-2

    Article  Google Scholar 

  • B Horstkotte O Elsholz V Cerdà (2005) J Flow Injection Anal 22 99 Occurrence Handle1:CAS:528:DC%2BD28XhsFOns7w%3D

    CAS  Google Scholar 

  • M A Segundo L M Magalhães (2006) Anal Sci 22 3 Occurrence Handle10.2116/analsci.22.3 Occurrence Handle1:CAS:528:DC%2BD28XntVyjtg%3D%3D

    Article  CAS  Google Scholar 

  • J Ruzicka E H Hansen (1988) Flow injection analysis EditionNumber2 John Wiley & Sons New York 23

    Google Scholar 

  • F R P Rocha B F Reis E A G Zagatto J L F C Lima R A S Lapa J L M Santos (2002) Anal Chim Acta 468 119 Occurrence Handle10.1016/S0003-2670(02)00628-1 Occurrence Handle1:CAS:528:DC%2BD38XmslGhtL4%3D

    Article  CAS  Google Scholar 

  • H Ukeda Y Adachi M Sawamura (2002) Talanta 58 1279 Occurrence Handle10.1016/S0039-9140(02)00411-3 Occurrence Handle1:CAS:528:DC%2BD38Xosl2ht7s%3D

    Article  CAS  Google Scholar 

  • H Ukeda (2004) Bunseki Kagaku 53 221 Occurrence Handle10.2116/bunsekikagaku.53.221 Occurrence Handle1:CAS:528:DC%2BD2cXjtlGmsrg%3D

    Article  CAS  Google Scholar 

  • M Polasek P Skala L Opletal L Jahodar (2004) Anal Bioanal Chem 379 754 Occurrence Handle1:CAS:528:DC%2BD2cXntValsrw%3D

    CAS  Google Scholar 

  • L M Magalhães M A Segundo S Reis J L F C Lima (2006) Anal Chim Acta 558 310 Occurrence Handle10.1016/j.aca.2005.11.013

    Article  Google Scholar 

  • D D Perrin B Dempsey (1979) Buffers for pH and metal ion control Chapman and Hall London 153

    Google Scholar 

  • M A Segundo H M Oliveira J L F C Lima M I G S Almeida A O S S Rangel (2005) Anal Chim Acta 537 207 Occurrence Handle10.1016/j.aca.2005.01.009 Occurrence Handle1:CAS:528:DC%2BD2MXjtl2lsrk%3D

    Article  CAS  Google Scholar 

  • P Goupy C Dufour M Loonis O Dangles (2003) J Agric Food Chem 51 615 Occurrence Handle10.1021/jf025938l Occurrence Handle1:CAS:528:DC%2BD38XpsFCnsLY%3D

    Article  CAS  Google Scholar 

  • W Brand-Williams M E Cuvelier C Berset (1995) Lebensm Wiss Technol 28 25 Occurrence Handle1:CAS:528:DyaK2MXjvV2itLw%3D

    CAS  Google Scholar 

  • Y Senba T Nishishita K Saito H Yoshioka H Yoshioka (1999) Chem Pharm Bull 47 1369 Occurrence Handle1:CAS:528:DyaK1MXmslCqsLk%3D

    CAS  Google Scholar 

  • C Sánchez-Moreno J A Larrauri F Saura-Calixto (1999) Food Res Int 32 407 Occurrence Handle10.1016/S0963-9969(99)00097-6

    Article  Google Scholar 

  • O Dangles G Fargeix C Dufour (1999) J Chem Soc, Perkin Trans 2 1387

    Google Scholar 

  • M C Foti C Daquino C Geraci (2004) J Org Chem 69 2309 Occurrence Handle10.1021/jo035758q Occurrence Handle1:CAS:528:DC%2BD2cXhsFyhsbs%3D

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. REQUIMTE, Serviço de Química-Física, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha 164, P-4099-030, Porto, Portugal

    Luís M. Magalhães, Marcela A. Segundo, Christophe Siquet, Salette Reis & José L. F. C. Lima

Authors
  1. Luís M. Magalhães
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marcela A. Segundo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Christophe Siquet
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Salette Reis
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. José L. F. C. Lima
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marcela A. Segundo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Magalhães, L., Segundo, M., Siquet, C. et al. Multi-syringe flow injection system for the determination of the scavenging capacity of the diphenylpicrylhydrazyl radical in methanol and ethanolic media. Microchim Acta 157, 113–118 (2007). https://doi.org/10.1007/s00604-006-0633-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00604-006-0633-7

Search

Navigation