Abstract
Purpose
Molecular markers as indicators for gastric cancer recurrence are urgently required. The aim of this study was to identify lectins that can be used to predict gastric cancer recurrence after gastrectomy.
Methods
We created lectin expression profiles by microarray analysis for 60 patients, who underwent surgery for gastric cancer at the Oita University Hospital between January, 2005 and December, 2007. Lectin expression and clinicopathological factors in patients who suffered gastric cancer recurrence and those who did not were compared by univariate and multivariate analyses.
Results
Thirteen lectins showed a significant increase in binding to cancer tissues, whereas 11 lectins showed a significant decrease in binding to cancer tissues, when compared with binding to normal epithelia. Multivariate analysis revealed that lymph node metastasis and low Bauhinia purpurea lectin (BPL)-binding signals were independent predictive factors for recurrence. All patients with low BPL expression had significantly worse relapse-free survival than those with high BPL expression.
Conclusions
Our results using a novel lectin microarray system provide the first solid evidence that BPL expression is a predictor of gastric cancer recurrence.
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Abbreviations
- CEA:
-
Carcinoembryonic antigen
- CA19-9:
-
Carbohydrate antigen 19-9
- HER2:
-
Human epidermal growth factor receptor 2
- EGFR:
-
Epidermal growth factor receptor
- CD44:
-
Cluster of differentiation 44
- ROC:
-
Receiver operating characteristic
- LCA:
-
Lens culinaris agglutinin
- MAL-I:
-
Maackia amurensis lectin I
- SNA:
-
Sambucus nigra agglutinin
- SSA:
-
Sambucus sieboldiana agglutinin
- TJA-I:
-
Trichosanthes japonica agglutinin I
- NPA:
-
Narcissus pseudonarcissus lectin
- ConA:
-
Concanavalin A
- GNA:
-
Galanthus nivalis agglutinin
- HHL:
-
Hippeastrum hybrid lectin
- ACG:
-
Agrocybe cylindracea galectin
- TxLC-I:
-
Tulipa gesneriana I
- MAH:
-
Maackia amurensis hemagglutinin
- UEA-I:
-
Ulex europaeus agglutinin I
- AOL:
-
Aspergillus oryzae lectin
- AAL:
-
Aleuria aurantia lectin
- BPL:
-
Bauhinia purpurea lectin
- EEL:
-
Euonymus europaeus lectin
- ABA:
-
Agaricus bisporus agglutinin
- WFA:
-
Wisteria floribunda lectin
- ACA:
-
Amaranthus caudatus agglutinin
- DBA:
-
Dolichos biflorus agglutinin
- SBA:
-
Soybean agglutinin
- GSL-I-A4:
-
Griffonia simplicifolia
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Acknowledgments
This study was supported, in part, by Grants-in-Aid for Scientific Research from the Japanese Society for the Promotion of Science (24591953). We thank Ms. Mayumi Takeda, Ms. Hiroko Taguchi, Ms. Yuiko Aso, and Ms. Maki Fujinaga for technical assistance.
Conflict of interest
T. Futsukaichi and his coauthors declare no conflicts of interest.
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Futsukaichi, T., Etoh, T., Nakajima, K. et al. Decreased expression of Bauhinia purpurea lectin is a predictor of gastric cancer recurrence. Surg Today 45, 1299–1306 (2015). https://doi.org/10.1007/s00595-015-1127-1
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DOI: https://doi.org/10.1007/s00595-015-1127-1