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Decreased expression of Bauhinia purpurea lectin is a predictor of gastric cancer recurrence

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Abstract

Purpose

Molecular markers as indicators for gastric cancer recurrence are urgently required. The aim of this study was to identify lectins that can be used to predict gastric cancer recurrence after gastrectomy.

Methods

We created lectin expression profiles by microarray analysis for 60 patients, who underwent surgery for gastric cancer at the Oita University Hospital between January, 2005 and December, 2007. Lectin expression and clinicopathological factors in patients who suffered gastric cancer recurrence and those who did not were compared by univariate and multivariate analyses.

Results

Thirteen lectins showed a significant increase in binding to cancer tissues, whereas 11 lectins showed a significant decrease in binding to cancer tissues, when compared with binding to normal epithelia. Multivariate analysis revealed that lymph node metastasis and low Bauhinia purpurea lectin (BPL)-binding signals were independent predictive factors for recurrence. All patients with low BPL expression had significantly worse relapse-free survival than those with high BPL expression.

Conclusions

Our results using a novel lectin microarray system provide the first solid evidence that BPL expression is a predictor of gastric cancer recurrence.

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Abbreviations

CEA:

Carcinoembryonic antigen

CA19-9:

Carbohydrate antigen 19-9

HER2:

Human epidermal growth factor receptor 2

EGFR:

Epidermal growth factor receptor

CD44:

Cluster of differentiation 44

ROC:

Receiver operating characteristic

LCA:

Lens culinaris agglutinin

MAL-I:

Maackia amurensis lectin I

SNA:

Sambucus nigra agglutinin

SSA:

Sambucus sieboldiana agglutinin

TJA-I:

Trichosanthes japonica agglutinin I

NPA:

Narcissus pseudonarcissus lectin

ConA:

Concanavalin A

GNA:

Galanthus nivalis agglutinin

HHL:

Hippeastrum hybrid lectin

ACG:

Agrocybe cylindracea galectin

TxLC-I:

Tulipa gesneriana I

MAH:

Maackia amurensis hemagglutinin

UEA-I:

Ulex europaeus agglutinin I

AOL:

Aspergillus oryzae lectin

AAL:

Aleuria aurantia lectin

BPL:

Bauhinia purpurea lectin

EEL:

Euonymus europaeus lectin

ABA:

Agaricus bisporus agglutinin

WFA:

Wisteria floribunda lectin

ACA:

Amaranthus caudatus agglutinin

DBA:

Dolichos biflorus agglutinin

SBA:

Soybean agglutinin

GSL-I-A4:

Griffonia simplicifolia

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Acknowledgments

This study was supported, in part, by Grants-in-Aid for Scientific Research from the Japanese Society for the Promotion of Science (24591953). We thank Ms. Mayumi Takeda, Ms. Hiroko Taguchi, Ms. Yuiko Aso, and Ms. Maki Fujinaga for technical assistance.

Conflict of interest

T. Futsukaichi and his coauthors declare no conflicts of interest.

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Correspondence to Tsuyoshi Etoh.

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Futsukaichi, T., Etoh, T., Nakajima, K. et al. Decreased expression of Bauhinia purpurea lectin is a predictor of gastric cancer recurrence. Surg Today 45, 1299–1306 (2015). https://doi.org/10.1007/s00595-015-1127-1

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