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Imaging in experimental models of diabetes

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Abstract

Translational medicine, experimental medicine and experimental animal models, in particular mice and rats, represent a multidisciplinary field that has made it possible to achieve, in the last decades, important scientific progress. In this review, we have summarized the most frequently used imaging animal models, such as ultrasound (US), micro-CT, MRI and the optical imaging methods, and their main implications in diagnostic and therapeutic fields, with a particular focus on diabetes mellitus, a multifactorial disease extremely widespread among the general population.

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Authors and Affiliations

  1. Diagnostic and Interventional Radiology Unit, ASST Settelaghi, Varese, Italy

    Andrea Coppola, Filippo Piacentino, Valeria Bettoni, Federico Fontana & Massimo Venturini

  2. Insubria University, Varese, Italy

    Giada Zorzetto, Filippo Piacentino, Giulio Carcano, Federico Fontana & Massimo Venturini

  3. Division of Endocrinology, ASST Fatebenefratelli-Sacco, Milan, Italy

    Ida Pastore

  4. Department of Diagnostic Radiology, Giovanni XXIII Hospital, Milano-Bicocca University, Bergamo, Italy

    Paolo Marra

  5. Experimental Imaging Center, San Raffaele Scientific Institute, Milan, Italy

    Laura Perani & Antonio Esposito

  6. Radiology Unit, San Raffaele Scientific Institute, San Raffaele Vita-Salute University, Milan, Italy

    Antonio Esposito & Francesco De Cobelli

  7. General, Emergency, and Transplant Surgery Unit, ASST Settelaghi, Varese, Italy

    Giulio Carcano

  8. International Center for T1D, Centro di Ricerca Pediatrica Romeo ed Enrica Invernizzi, Dipartimento di Scienze Biomediche e Cliniche “L. Sacco”, Università di Milano, Milan, Italy

    Paolo Fiorina

  9. Nephrology Division, Boston Children’s Hospital, Harvard Medical School, Boston, MA, USA

    Paolo Fiorina

  10. Endocrinology Division, ASST Fatebenefratelli Sacco, Milan, Italy

    Paolo Fiorina

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  1. Andrea Coppola
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592_2021_1826_MOESM1_ESM.gif

Supplementary Animated Figure S1. Ultrasound imaging of the pancreas of a RIP-Tag transgenic mouse expressing the oncogenic SV40 large T antigen (Tag) under the transcriptional control of the insulin promoter, obtained with a 40 MHz probe. Multiple round hypoechoic pancreatic lesions (annotations) can be seen, consistent with pancreatic cancer (β-islet tumors) (GIF 47 kb)

Supplementary Video S2. Ultrasound imaging of the right kidney in a diabetic murine model. The kidney cortex is markedly hyperechoic if compared to hepatic parenchyma (mov 4721 kb)

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Coppola, A., Zorzetto, G., Piacentino, F. et al. Imaging in experimental models of diabetes. Acta Diabetol 59, 147–161 (2022). https://doi.org/10.1007/s00592-021-01826-3

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