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MicroRNAs modulation and clinical outcomes at 1 year of follow-up in obese patients with pre-diabetes treated with metformin vs. placebo

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Abstract

Backgrounds

Obese pre-diabetics over express cytokines that influence myocardial function via microRNAs (miRs) expression.

Objectives

To evaluate inflammatory/oxidative stress, miRs’ expression and cardiovascular function in obese pre-diabetics assigned to metformin therapy vs. placebo vs. normo-glycemics at 12 months of follow-up.

Materials and methods

Eighty-three obese patients after abdominoplastic surgery were divided in pre-diabetics (n 55), normo-glycemics (n 28), and assigned to hypocaloric diet. Pre-diabetics were assigned to metformin (n 23) or to placebo (n 22) plus hypocaloric diet.

Results

Obese pre-diabetics in metformin vs. placebo, and obese pre-diabetics with placebo vs. normoglycemics, had significant differences about IMT, MPI, and LVM (p < 0.05). Obese pre-diabetics in metformin vs. placebo showed significant reduction in serum miR-195 and miR-27 (p < 0.05). Obese pre-diabetics in metformin vs. normoglycemics showed higher expression of serum miR-195 and miR-27 (p < 0.05). Finally, we found inverse relation between IMT and insulin, HOMA-IR, miR-195, miR-27; between LVEF and Insulin, HOMA-IR, miR-195 and miR-27. We found inverse correlation between LVM and sirtuin-1, Insulin, HOMA-IR, miR-195 and miR-27, and direct correlation with interleukin-6. MPI inversely linked to miR-195 and miR-27.

Conclusions

In obese pre-diabetics’, metformin significantly reduces inflammation/oxidative stress, and miR-195 and miR-27, with reduction in LVM, IMT.

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Acknowledgements

CS contributed to study design, statistical analysis, manuscript writing; GPA, GFN and RM contributed to study design; GPI, and GF contributed to surgical interventions; GG contributed to data interpretation and study revision; MCT, MDA performed experiments and miRs analysis; F.Fe, I. P performed hematoxylin and eosin-stained sections.

Funding

None to declare.

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Authors and Affiliations

  1. Department of Medical, Surgical, Neurological, Metabolic and Aging Sciences, University of Campania “Luigi Vanvitelli”, Piazza Miraglia 2, 80138, Naples, Italy

    Celestino Sardu, Angela Abbatecola, Giuseppe Paolisso & Raffaele Marfella

  2. Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Maria Consiglia Trotta, Gianluca Gatta, Nunzia D’ Onofrio, Maria Luisa Balestrieri, Michele D’ Amico, Franca Ferraraccio & Iacopo Panarese

  3. Department of Plastic Surgery, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Gorizio Pieretti, Giuseppe Ferraro & Giovanni Francesco Nicoletti

  4. Department of Radiology, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Gianluca Gatta

  5. Mediterranea Cardiocentro, Naples, Italy

    Giuseppe Paolisso & Raffaele Marfella

Authors
  1. Celestino Sardu
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  2. Maria Consiglia Trotta
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  8. Maria Luisa Balestrieri
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  9. Michele D’ Amico
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  14. Raffaele Marfella
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Corresponding author

Correspondence to Celestino Sardu.

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Human and animal rights

Authors indicate the procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (University of Campania “Luigi Vanvitelli”, Naples, Italy).

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Study participants gave the written consent to participate in the study.

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Sardu, C., Trotta, M.C., Pieretti, G. et al. MicroRNAs modulation and clinical outcomes at 1 year of follow-up in obese patients with pre-diabetes treated with metformin vs. placebo. Acta Diabetol 58, 1381–1393 (2021). https://doi.org/10.1007/s00592-021-01743-5

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  • DOI: https://doi.org/10.1007/s00592-021-01743-5

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