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Enhanced syndecan-1 expression on neutrophils in patients with type 2 diabetes mellitus

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Abstract

The peripheral neutrophils are one of the main inflammatory cells and significantly influence the damage of endothelium. Type 2 diabetes is a manifestation of an ongoing low-grade inflammation. In diabetes, impairment of neutrophil adhesion to the endothelium and migration to the site of inflammation were detected, which associated closely with adhesion molecules expressed on neutrophils and endothelial cells. To detect the expression of syndecan-1 on neutrophils in patients with type 2 diabetes mellitus, we recruited 29 patients with type 2 diabetes mellitus without any diabetic complication and 24 healthy subjects (controls). Expression of syndecan-1 was determined by flow cytometry, and potential correlations between syndecan-1 and clinical characteristics were analyzed. On neutrophils, percentage of positive syndecan-1 cells was significantly higher in subjects with diabetes (10.363 ± 1.689%) than that of the controls (3.775 ± 0.634%, P = 0.001). An association between body mass index (BMI) and percentage of positive syndecan-1 neutrophils was detected (r = 0.415, P = 0.025). When BMI was categorized into subgroups of ≤25 kg/m2 (n = 10) and >25 kg/m2 (n = 19), the average percentages of positive syndecan-1 neutrophils in patients with diabetes were 5.733 ± 1.842% and 12.642 ± 2.251%, respectively (t = −2.137, P = 0.042). A multiple regression analysis showed that BMI (β = 0.783, P < 0.000) was a significant predictor of positive syndecan-1 neutrophils in subjects with type 2 diabetes.

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Acknowledgments

We are grateful to the following nurses for their help in collecting the peripheral venous blood samples: Li Yang, Shi-Shi Li, Jing Zhao, Ze Zhang, Dan-Dan Li, Chun-Mei Zhang, Ying-Qin Xie, Ya-Li Liu, Li-Hong Zhou, Li-Bo Yang, Shuang Guo.

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Authors and Affiliations

  1. Department of Ophthalmology, The 309th Hospital of Chinese People’s Liberation Army, Jia 17, Heishanhu Road, Haidian District, 100091, Beijing, China

    Jing-Bo Wang, Juan Guan, Li Zhou, Yu Sheng & Yan-Fang Si

  2. College of Pharmacy, University of Cincinnati Academic Health Center, 3225 Eden Ave, 136 HPB, Cincinnati, OH, 45267-0004, USA

    Yan-Jun Zhang

  3. Department of Endocrinology, The 309th Hospital of Chinese People’s Liberation Army, Jia 17, Heishanhu Road, Haidian District, 100091, Beijing, China

    Yan Zhang

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  1. Jing-Bo Wang
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  6. Yan Zhang
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Correspondence to Jing-Bo Wang.

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Wang, JB., Zhang, YJ., Guan, J. et al. Enhanced syndecan-1 expression on neutrophils in patients with type 2 diabetes mellitus. Acta Diabetol 49, 41–46 (2012). https://doi.org/10.1007/s00592-011-0265-1

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  • DOI: https://doi.org/10.1007/s00592-011-0265-1

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