Skip to main content
SpringerLink
Log in

The comparison of pathophysiological effects of the extract of Trigonella and Galega plants with metformin on some biochemical parameters in streptozotocin-induced diabetic rats

  • Original Article
  • Published:
Comparative Clinical Pathology Aims and scope Submit manuscript

Abstract

Studies show that the extract of Trigonella and Galega plants causes a significant decrease in glucose levels in diabetic animals. The aim of this study is to compare the effects of the extract of Trigonella and Galega plants with metformin in improving diabetes complications on some biochemical parameters in diabetic rats. A total of 70 adult male Wistar rats were randomly treated in the following groups for 1 month (control group, diabetic, diabetic + extract of Trigonella (1.5 and 3 g/kg), diabetic the extract of Galega (1.5 and 3 g/kg), diabetic + metformin (250 mg/kg)). At the beginning and at the end of the experiment, the rats were weighed and their blood glucoses were measured, and then the rats were bled to measure enzymes of ALT, HDL, LDL, triglycerides (TG), blood urea nitrogen (BUN), T.Billi, ALP, uric acid, creatinine, and ASt. After obtaining the serum, the values of the above parameters were measured and then the data were analyzed using SPSS 18 software and Duncan’s statistical test. The results indicate a significant increase in blood glucose, TG, and BUN and a significant decrease in albumin and HDL in the diabetic group in comparison to the control group (p ≤ 0.05). Also, the plant extracts and metformin caused a reduction in diabetes complications. The results of this study indicate that Trigonella plant with a dose of 1.5 g/kg of the body weight and metformin improve the diabetic complications on biochemical parameters better than does the Galega plant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Abdel-Barry JA, Abdel-Hassan IA, Jawad AM, Al-Hakiem MH (2000) Hypoglycaemic effect of aqueous extract of the leaves of Trigonella foenum-graecum in healthy volunteers. East Mediterr Health J 6(1):83–88

    CAS  PubMed  Google Scholar 

  • Almdal JP, Vilstrup H (1988) Strict insulin therapy normalize organ nitrogen contents capacity of urea nitrogen synthesis in experimental diabetes in rats. Diabetlogia 31:114–118

    Article  CAS  Google Scholar 

  • Anitha Devi B, Kamalakkannan N, Stanely Mainzen Prince P (2003) Supplementation of fenugreek leaves to diabetic rats: effect on carbohydrate metabolic enzymes in diabetic liver and kidney. Phytother Res 17:1231–1233

    Article  PubMed  Google Scholar 

  • Asgary S, Moshtaghian SJ, Setorki M, Kazemi S, Rafieian-kopaei M, Adelnia A (2011) Hypoglycaemic and hypolipidemic effects of pumpkin (Cucurbita pepo L.) on alloxan-induced diabetic rats. Afr J Pharm Pharmacol 5(23):2620–2626

    CAS  Google Scholar 

  • Azarnia M, Minaee B, Mozafari Z, Tahamtan Y (2009) Histopathological effects of fenugreek leaf extract (Trigonella foenum-graecum) on rat fetal long bone development. J Develop Biol 1:50–58

    Google Scholar 

  • Blumenthal M, Goldberg A, Brinckmann J (2000) Herbal Medicine Integrative Medicine Communications. Austin 8:401

    Google Scholar 

  • Cheng JT, Huang CC, Liu IM, Tzeng TF, Chang CJ (2006) Novel mechanism for plasma glucose-lowering action of metformin in streptozotocin-induced diabetic rats. Diabetes 55(3):819–825

    Article  CAS  PubMed  Google Scholar 

  • Farhad K, Hossein KJ, Shahla Z, Sara A, Mohammad F, Saeid MT (2013) The effect of hydroalcoholic extract of artemisia (Artemisia herba-alba) and markers of liver damage compared with metformin in streptozotocin-induced diabetic rats. Adv Environ Biol 7(4):743–748

    CAS  Google Scholar 

  • Halliwell B, Gutteridge JMC (1989) Free radicals in biology and medicine, vol 43, 2nd edn. Clarendon, Oxford, pp 186–189

    Google Scholar 

  • Hayashi T, Nozawa M, Sohmiya K, Toko H, Nakao M, Okabe M et al (1998) E fficacy of pancreatic transplantation on cardiovascular alterations in diabetic rats: an ultrastructural and immunohistochemical study. Transpl Proc 30:335–338

    Article  CAS  Google Scholar 

  • Hostetter TH (1992) Diabetic nephropathy: methabolic versus hemodynamic considerations. Diabetes Care 15:1205–1215

    Article  CAS  PubMed  Google Scholar 

  • Huang C, Fornage M, Lloyd-Jones DM et al (2004) Mitochondrial substitution rates are extraordinarily elevated and variable iHhin a genus of flowering plants. Proc Natl Acad Sci U S A 101(51):17741–17746

    Article  Google Scholar 

  • Kato A, Minoshima Y, Yamamoto J, Adachi I, Alison Watson A, Robert Nash J (2008) Protective effects of dietary chamomile tea on diabetic complications. J Agric Food Chem 56:17

    Google Scholar 

  • Kume E, Fujimura H, Matsuki N, Ito M, Aruga C, Toriumi W (2004) Hepatic changes in the acute phase of streptozotocin-induced diabetes in mice. Exp Toxic Pathol 55:467–480

    Article  Google Scholar 

  • Luka CD, Omoniwa BP (2012) Effect of some phytochemicals extracted from goat’s rue (Galega officinalis) on some biochemical parameters in normal and alloxan-induced diabetic rats. J Nat Prod Plant Resour 2(5):628–632

    CAS  Google Scholar 

  • Meyer DJ, Coles EH, Rich LJ (1992) Veterinary laboratory medicine: interpretation and diagnosis, 1st edn. Saunders, Philadelphia, pp 84–86

    Google Scholar 

  • Oyedemi SO, Bradly G, Afolayan AJ (2011) Beneficial effect ofaqueous stem bark extract of Strychnos henningsii Gilg in streptozotocin-nicotinamide induced type 1 diabetic Wistar rats. Int J Pharm 7(7):773–781

    Article  Google Scholar 

  • Rashki Kemmak M, Gol A, Dabiri SH (2009) Preventive effects of garlic juice on renal damages induced by diabetes mellitus in rats. Iran J Endocrinol Metab 11(3):331–340

    Google Scholar 

  • Roghani, Mehrdad, Balouch Nejhad Mojarrad, Toorandokht, and Roghani Dehkordi, Farshad (1384), Hypolipidemic effect of the water extract of Trigonella leaves in diabetic rats, Iranian Journal of Endocrine and Metabolism, University of Medical Sciences and Health Services of Shahid Beheshti, 7 2 pp: 167-171

  • Sauvaire Y, Baissac Y, Leconte O, Petit P, Ribes G (1996) Steroid saponins from fenugreek and some of their biological properties. Adv Exp Med Biol 405:37–46

    Article  CAS  PubMed  Google Scholar 

  • Tappy L, Minehira K (2001) New data and new concepts on the role of the liver in glucose homeostasis. Curr Opin Clin Nutr Metab Care 4:273–277

    Article  CAS  PubMed  Google Scholar 

  • Thomson M, AI-Amin ZM, AI-Qattan KK, Shaban LH, Muslim A (2007) Anti-diabetic and hypolipidemic properties of garlic Allium sativum in streptozotpcin-induced rats. Int J Diab Metab 15:108–115

    Google Scholar 

  • Varshney IP, Sharma SC (1996) Saponins XXXII Trigonella foenum graecum seeds. J Indian Chem Soc 43:564–567

    Google Scholar 

  • Zia T, Hasnain SN, Hasan SK (2001) Evaluation of the oral hypoglycaemic effect of Trigonella foenum-graceum L (methi) in normal mice. J Ethnopharmacol 75:191–197

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors wish to express their sincere thanks to Endocrine and Metabolism Research Center, Namazi Hospital, Shiraz, I.R, Iran, for their cooperation in this study.

Author information

Authors and Affiliations

  1. Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran

    Seyedeh Sahar Shojaei & Hossein Kargar Jahromi

  2. Department of Biology, Fars Science and Research Branch, Islamic Azad University Marvdasht Iran, Shiraz, Iran

    Seyedeh Sahar Shojaei

  3. Young Researchers Club Elite, Jahrom Branch, Islamic Azad University, Jahrom, Iran

    Hossein Kargar Jahromi

  4. Endocrine and Metabolism Research Center, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Islamic Republic of Iran

    Farhad Koohpeyma

  5. Institution of Supreme Education and Industry, Maragheh, Iran

    Seyed Hamid Bathaee

  6. Department of Anatomy and Embryology, International Branch, Shiraz University, Shiraz, Iran

    Mohammad Farzam

  7. Fars Science and Research Branch, Islamic Azad University, Marvdasht, Iran

    Seyedeh Sahar Shojaei

Authors
  1. Seyedeh Sahar Shojaei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hossein Kargar Jahromi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Farhad Koohpeyma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Seyed Hamid Bathaee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mohammad Farzam
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Seyedeh Sahar Shojaei.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shojaei, S.S., Jahromi, H.K., Koohpeyma, F. et al. The comparison of pathophysiological effects of the extract of Trigonella and Galega plants with metformin on some biochemical parameters in streptozotocin-induced diabetic rats. Comp Clin Pathol 24, 303–309 (2015). https://doi.org/10.1007/s00580-014-1899-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00580-014-1899-5

Keywords

Search

Navigation