Abstract
The prevalence of canine microcytosis, detected as reduced mean corpuscolar volume (MCV) by impedance cell counter was investigated retrospectively in relationship to sex, breed, diseases, and other blood count parameters. One thousand and twelve (1,012) canine medical records with complete blood count (CBC) were evaluated for MCV results. CBCs were performed using impedance cell counter and stained blood smears for leukocyte differential count, erythrocyte morphology, and platelet estimation. Statistical analysis included both comparative and descriptive investigations in dogs showing microcytosis (MICRO) versus dogs with normal MCV (control, CTR). MCV was lower than the reference range in 8.5% of medical records (86/1,012). Only 47.7% of MICRO dogs were affected by different degrees of anemia. In MICRO dogs, thrombocytosis was present in 19.8%, while 16.3% showed thrombocytopenia, and mild leukocytosis was found in 30.2%. Statistical analysis showed significant difference about sex and breeds in MICRO vs. CTR dogs while different disorders did not affect the MICRO vs. CTR dogs; few CBC parameters such as erythrocyte count, hematocrit, and red cell dimension width were both significantly related to MCV of MICRO dogs and between CTR and MICRO dogs. Multivariate hierarchical cluster analysis showed significant difference among clusters considering platelets and displayed a subgroup of patients in MICRO dogs. Microcytosis detected at low rate in CBC of clinical records is not always related to anemia. The comparison in MICRO and CTR dogs showed a significant difference for most blood count parameters dealing with erythrocytes and leukocytes but not for platelets.
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StaticXL 1.7 beta software
References
Aslan D, Altay C (2003) Incidence of high erythrocyte count in infants and young children with iron deficiency anemia: re-evaluation of an old parameter. J Pediatr Hematol Oncol 25(4):303–306
Bunch SE, Jordan HL, Sellon RK, Cullen JM, Smith JE (1995) Characterization of iron status in young dogs with portosystemic shunt. Am J Vet Res 56(7):853–858
Brockus CW, Andreasen CB (2003) Erythrocytes. In: Latimer KS, Mahaffey EA, Prasse KW (eds) Clinical pathology, 4th edn. Iowa State Press, Iowa, pp 3–45
Dan K (2005) Thrombocytosis in iron deficiency anemia. Intern Med 44(10):1025–1026
Degen M (1987) Pseudohyperkalemia in Akitas. J Am Vet Med Assoc 190(5):541–543
Fry MM (2010) Anemia of inflammatory, neoplastic, renal, and endocrine diseases. In: Weiss DJ, Wardrop KJ (eds) Schalm’s veterinary hematology, 6th edn. Wiley-Blackwell, Ames, pp 246–250
Goodfellow M, Papasouliotis K, Cue S, Crawford E, Hall E (2008) Effect of storage on microcytosis observed in dogs with portosystemic vascular anomalies. Res Vet Sci 84(3):490–493
Gookin JL, Bunch SE, Rush LJ, Grindem CB (1998) Evaluation of microcytosis in 18 Shibas. J Am Vet Med Assoc 212(8):1285–1289
Harvey JW (2001) Hematopoiesis. In: Harvey JW (ed) Atlas of veterinary hematology, 1st edn. WB Saunders, Philadelphia, pp 87–91
Harvey JW, Coleman MW (1982) Iron deficiency anemia in a Siberian lynx. J Am Vet Med Assoc 181(11):1402–1403
Kadikoylu G, Yavasoglu I, Bolaman Z, Senturk T (2006) Platelet parameters in women with iron deficiency anemia. J Natl Med Assoc 3:398–402
Killip S, Bennet JM, Chambers MD (2007) Iron deficiency anemia. Am Fam Phys 75:671–678
Laflamme DP, Mahaffey EA, Allen SW, Twedt DC, Prasse KW, Huber TL (1994) Microcytosis and iron status in dogs with surgically induced portosystemic shunts. J Vet Intern Med 8(3):212–216
Marchetti V, Lubas G, Scarpa M, Luchetti E (2006) Haematological modifications in dogs and cats with chronic gastroenteritis. Soc It Scie Vet Proc LX:253–254
Marchetti V, Lubas G, Lombardo A, Corazza M, Guidi G, Cardini G (2010) Evaluation of erythrocytes, platelets, and serum iron profile in dogs with chronic enteropathy. Vet Med Intl. doi:10.4061/2010/716040
Martelli A, Bernabò N, Berardinelli P, Russo V, Rinaldi C, Di Giacinto O, Mauro A, Barboni B (2009) Vascular supply as a discriminating factor for pig preantral follicle selection. Reproduction 137(1):45–58
Meyer DJ, Harvey JW (1994) Hematologic changes associated with serum and hepatic iron alterations in dogs with congenital portosystemic vascular anomalies. J Vet Intern Med 8(1):55–56
Paltrinieri S, Preatoni M, Rossi S (2010) Microcytosis does not predict serum iron concentrations in anaemic dogs. Vet J 185(3):341–343
Perret D, Trumel C, Diquélou A, Dossin O, Guelfi JF, Espie C (2001) L’indice de distribution des globules rouges (IDR) chez le chien. Analyse de 1400 cas. Rev Med Vet 152(7):549–554
Rajantie J (2006) Erythrocyte count is often normal or elevated in children with iron deficiency anemia. J Pediatr Hematol Oncol 28(8):547
Schafer AI (2006) Essential thrombocythemia and thrombocytosis. In: Lichtman MA, Beutler E, Kipps TJ, Seligsohn U, Kaushansky K, Prchal JT (eds) Williams hematology, 7th edn. McGraw-Hill, New York, pp 1785–1794
Simpson KW, Meyer DJ, Boswood A, White RN, Maskell IE (1997) Iron status and erythrocyte volume in dogs with congenital portosystemic vascular anomalies. J Vet Intern Med 11(1):14–19
Steinberg JD, Olver CS (2005) Hematologic and biochemical abnormalities indicating iron deficiency are associated with decreased reticulocyte hemoglobin content (CHr) and reticulocyte volume (rMCV) in dogs. Vet Clin Pathol 34:23–27
Thomas C, Thomas L (2002) Biochemical markers and hematologic indices in the diagnosis of functional iron deficiency. Clin Chem 48(7):1066–1197
Villiers E (2005) Introduction to haematology. In: Villiers E, Blackwood L (eds) BSAVA manual of canine and feline clinical pathology, 2nd edn. Wiley-Blackwell, London, pp 37–63
Walker D (2008) Peripheral blood smears. In: Cowell RD, Tyler RD, Meinkoth JH, DeNicola DB (eds) Diagnostic cytology and hematology of the dog and cat, 3rd edn. Mosby Elsevier, St Louis, pp 390–421
Weiss JD (2010) Iron and copper deficiencies and disorders of iron metabolism. In: Weiss DJ, Wardrop KJ (eds) Schalm’s veterinary hematology, 6th edn. Wiley-Blackwell, Ames, pp 167–171
Weiss JD, Tvedten H (2004) Erythrocyte disorders. In: Willard MD, Tvedten H (eds) Small animal clinical diagnosis by laboratory methods, 4th edn. WB Saunders, Missouri, pp 38–62
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Gavazza, A., Rispoli, D., Bernabò, N. et al. Retrospective and observational investigation of canine microcytosis in relationship to sex, breed, diseases, and other complete blood count parameters. Comp Clin Pathol 21, 545–553 (2012). https://doi.org/10.1007/s00580-010-1127-x
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DOI: https://doi.org/10.1007/s00580-010-1127-x