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Water management, rice varieties and mycorrhizal inoculation influence arsenic concentration and speciation in rice grains

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Abstract

A pot experiment was carried out to investigate the effects of water management and mycorrhizal inoculation on arsenic (As) uptake by two rice varieties, the As-resistant BRRI dhan 47 (B47) and As-sensitive BRRI dhan 29 (B29). Grain As concentration of B47 plants was significantly lower than that of B29, and grain As concentration of B47 was higher under flooding conditions than that under aerobic conditions. In general, mycorrhizal inoculation (Rhizophagus irregularis) had no significant effect on grain As concentrations, but decreased the proportion of inorganic arsenic (iAs) in grains of B47. The proportion of dimethylarsinic acid (DMA) in the total grain As was dramatically higher under flooding conditions. Results demonstrate that rice variety selection and appropriate water management along with mycorrhizal inoculation could be practical countermeasures to As accumulation and toxicity in rice grains, thus reducing health risks of As exposure in rice diets.

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Abbreviations

AMF:

Arbuscular mycorrhizal fungi

As:

Arsenic

As(III):

Arsenite

As(V):

Arsenate

B29:

BRRI dhan 29

B47:

BRRI dhan 47

DMA:

Dimethylarsinic acid

HPLC-ICP-MS:

High performance liquid chromatography-inductively coupled plasma-massspectrometry

iAs:

Inorganic arsenic

ICP-OES:

Inductively coupled plasma-optical emission spectrometer

ICP-MS:

Inductively coupled plasma-mass spectroscopy

MMA:

Monomethylarsonic acid

R. irregularis :

Rhizophagus irregularis

TFA:

Trifluoroacetic acid

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Acknowledgments

We thank Prof. M. Tofazzal Islam from Bangabandhu Sheikh Mujibur Rahman Agricultural University for kindly supplying the seeds of BRRI dhan 47 and BRRI dhan 29 for the investigation. This study was financially supported by the National Natural Science Foundation of China (41101246, 41471219) and the International Foundation for Science, Stockholm, Sweden, through a grant to Xin Zhang (NO. C/4973-1).

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    1. State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China

      Xin Zhang, Songlin Wu, Baihui Ren & Baodong Chen

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    1. Xin Zhang
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    2. Songlin Wu
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    3. Baihui Ren
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    4. Baodong Chen
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    Correspondence to Baodong Chen.

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    Xin Zhang and Songlin Wu contributed equally to this work.

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    Zhang, X., Wu, S., Ren, B. et al. Water management, rice varieties and mycorrhizal inoculation influence arsenic concentration and speciation in rice grains. Mycorrhiza 26, 299–309 (2016). https://doi.org/10.1007/s00572-015-0669-9

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