Skip to main content

Advertisement

SpringerLink
Log in

The combination of hydrogen gas and hydrogen-rich solution does not protect against ischemic spinal cord injury in rabbits

  • Original Article
  • Published:
Journal of Anesthesia Aims and scope Submit manuscript

Abstract

Purpose

This study aimed to determine whether the combination of H2 gas inhalation and administration of hydrogen-rich acetated Ringer’s solution (HS) could protect against ischemic spinal cord injury in rabbits.

Methods

In Experiment 1, rabbits were randomly assigned to a 1.2% H2 gas group, HS group, 1.2% H2 gas + HS group (combination group), or control group (n = 6 per group). The H2 concentration of HS was 0.65 mM. H2 was inhaled for 60 min, starting 5 min before reperfusion. HS (20 mL/kg) was divided into six bolus injections at 10-min intervals, starting 5 min before reperfusion. Spinal cord ischemia was produced by occluding the abdominal aorta for 15 min. Neurologic and histopathologic evaluations were performed 7 days after reperfusion. In Experiment 2, H2 concentrations in spinal cord tissue according to the administration of 1.2% H2 gas or HS were compared by measuring the electric current through a platinum needle electrode (n = 2). In Experiment 3, rabbits were assigned to a 2% H2 gas group or control group (n = 6 per group). Spinal cord ischemia was produced and neurologic and histopathologic evaluations were performed as in Experiment 1.

Results

There were no significant differences among the groups in the neurologic and histopathologic outcomes in Experiments 1 and 3. Bolus administration of HS (10 mL) transiently increased the current to only 1/30th and 1/27th of the plateau current with 1.2% H2 gas inhalation in two animals.

Conclusion

These results suggest that the combination of 1.2% H2 gas inhalation and administration of a hydrogen-rich solution does not protect against ischemic spinal cord injury and that the increase in H2 concentration in spinal cord tissue after administration of HS is very low compared to 1.2% H2 gas inhalation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Data availability

Data used in this paper may be submitted upon request.

References

  1. Ohsawa I, Ishikawa M, Takahashi K, Watanabe M, Nishimaki K, Yamagata K, Katsura K, Katayama Y, Asoh S, Ohta S. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med. 2007;13(6):688–94.

    Article  PubMed  CAS  Google Scholar 

  2. Ichihara M, Sobue S, Ito M, Ito M, Hirayama M, Ohno K. Beneficial biological effects and the underlying mechanisms of molecular hydrogen – comprehensive review of 321 original articles. Med Gas Res. 2015;5:12.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Huang Y, Xie K, Li J, Xu N, Gong G, Wang G, Yu Y, Dong H, Xiong L. Beneficial effects of hydrogen gas against spinal cord ischemia-reperfusion injury in rabbits. Brain Res. 2011;1378:125–36.

    Article  PubMed  CAS  Google Scholar 

  4. Zhou L, Wang X, Xue W, Xie K, Huang Y, Chen H, Gong G, Zeng Y. Beneficial effects of hydrogen-rich saline against spinal cord ischemia-reperfusion injury in rabbits. Brain Res. 2013;1517:150–60.

    Article  PubMed  CAS  Google Scholar 

  5. Kimura A, Suehiro K, Mukai A, Fujimoto Y, Funao T, Yamada T, Mori T. Protective effects of hydrogen gas against spinal cord ischemia-reperfusion injury. J Thorac Cardiovasc Surg. 2022;164(6):e269–83.

    Article  PubMed  Google Scholar 

  6. Utada K, Ishida K, Tohyama S, Urushima Y, Mizukami Y, Yamashita A, Uchida M, Matsumoto M. The combination of insulin-like growth factor 1 and erythropoietin protects against ischemic spinal cord injury in rabbits. J Anesth. 2015;29(5):741–8.

    Article  PubMed  Google Scholar 

  7. Fukui T, Ishida K, Mizukami Y, Shiramoto K, Harada H, Yamashita A, Yamashita S, Matsumoto M. Comparison of the protective effects of direct ischemic preconditioning and remote ischemic preconditioning in a rabbit model of transient spinal cord ischemia. J Anesth. 2018;32(1):3–14.

    Article  PubMed  Google Scholar 

  8. Matsumoto M, Iida Y, Sakabe T, Sano T, Ishikawa T, Nakakimura K. Mild and moderate hypothermia provide better protection than a burst-suppression dose of thiopental against ischemic spinal cord injury in rabbits. Anesthesiology. 1997;86(5):1120–7.

    Article  PubMed  CAS  Google Scholar 

  9. Drummond JC, Moore SS. The influence of dextrose administration on neurologic outcome after temporary spinal cord ischemia in the rabbit. Anesthesiology. 1989;70:64–70.

    Article  PubMed  CAS  Google Scholar 

  10. Ono H, Nishijima Y, Adachi N, Sakamoto M, Kudo Y, Nakazawa J, Kaneko K, Nakao A. Hydrogen(H2) treatment for acute erythematous skin diseases. A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers. Med Gas Res. 2012;2(1):14.

Download references

Funding

This study was partly supported by a grant for developing hydrogen engineering applications in medical and health care from Yamaguchi Prefecture.

Author information

Authors and Affiliations

  1. Department of Anesthesiology, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan

    Atsuo Yamashita, Satoshi Yamashita & Mishiya Matsumoto

  2. Department of Anesthesiology, NHO Kanmon Medical Center, Yamaguchi, Japan

    Takehiko Fukui

  3. Department of Anesthesiology, Kurashiki Central Hospital, Kurashiki, Japan

    Kazuyoshi Ishida

Authors
  1. Atsuo Yamashita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takehiko Fukui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Satoshi Yamashita
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kazuyoshi Ishida
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mishiya Matsumoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mishiya Matsumoto.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yamashita, A., Fukui, T., Yamashita, S. et al. The combination of hydrogen gas and hydrogen-rich solution does not protect against ischemic spinal cord injury in rabbits. J Anesth (2024). https://doi.org/10.1007/s00540-024-03334-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00540-024-03334-4

Keywords

Search

Navigation