Abstract
Background
The high mortality rate of severe acute pancreatitis (SAP) is closely associated with secondary infections of pancreatic and peripancreatic tissues. It was reported that granulocyte colony-stimulating factor (G-CSF) increased the number of leukocytes and enhanced their functions. However, an inflammatory response may be enhanced by an increased number of leukocytes. Our purpose was to study the roles of G-CSF in peritoneal-exudate neutrophils and inflammatory cytokines in the early stage of experimental SAP.
Methods
SAP was induced by injecting 0.2 ml of 3% taurocholate acid into the biliopancreatic duct in male Wistar rats. G-CSF (90 µg/kg body weight) or saline was administered 1 h before the SAP induction. The number of neutrophils and their phagocytic and bactericidal activities were evaluated, and the concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β in plasma and ascitic fluid were measured 1 h and 3 h after the SAP induction.
Results
The number of peritoneal-exudate neutrophils (PENs) at 3 h was increased by G-CSF administration (81 ± 50 × 105 cells/total exudate), as compared with that shown with saline administration (28 ± 13 × 105 cells/total exudate; P < 0.05). The numbers of phagocytic and bactericidal neutrophils were also elevated by G-CSF administration. G-CSF administration did not increase the concentrations of TNF-α, IL-6, and IL-1β in the plasma and ascitic fluid.
Conclusions
G-CSF increases the numbers of neutrophils and enhances their functions against bacteria, but it does not enhance intraabdominal and systemic inflammatory responses in the early stage of SAP.
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Tuo, H., Sugiyama, M., Nakashima, M. et al. Effects of granulocyte colony-stimulating factor on neutrophils and inflammatory cytokines in the early stage of severe acute pancreatitis in rats. J Gastroenterol 40, 186–191 (2005). https://doi.org/10.1007/s00535-004-1515-6
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DOI: https://doi.org/10.1007/s00535-004-1515-6