Abstract
Cryoimmobilization by high-pressure freezing, combined with cryosubstitution and resin embedding, allowed accurate retention in situ of the small (12–15 kDa) water-soluble protein, profilin, in anthers of Ledebouria socialis Roth (Hyacinthaceae). The subcellular distribution of profilin was investigated by using post-embedding immunogold labelling with rabbit antisera raised against recombinant birch profilin (RP2) or birch COOH-terminal profilin peptide (RP3). The patterns observed in mature pollen grains are novel to eukaryotic organisms: profilin was consistently demonstrated within both the vegetative and generative nuclei, an addition to its well-known presence in the cytoplasm. Methodological and immunological aspects, as well as possible biological implications, of this finding are considered.
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Received: 17 March 1997 / Revision accepted: 10 July 1997
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Hess, M., Valenta, R. Profilin revealed in pollen nuclei: immuno-electron microscopy of high-pressure frozen Ledebouria socialis Roth (Hyacinthaceae). Sex Plant Reprod 10, 283–287 (1997). https://doi.org/10.1007/s004970050099
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DOI: https://doi.org/10.1007/s004970050099