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A reliable protocol for direct detection of lectin binding sites on the plasma membrane of a single living sperm cell in maize

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Sexual Plant Reproduction Aims and scope Submit manuscript

Abstract.

A novel protocol for direct detection and localization of lectin binding sites on the surface of single sperm cells is presented. Fluorescence-conjugated lectins and single cell manipulation techniques were employed in this protocol. Advantages of the protocol include that sperm cell membranes are well protected and artifacts avoided by minimizing movement of the medium. Damage of the sperm membrane was reduced using a droplet-staining/washing method. Any morphological change may be readily recorded.

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Authors and Affiliations

  1. The key laboratory of MOE for Developmental Biology, Wuhan University, Wuhan 430072, PR China, , , , ,

    Meng-Xiang Sun & Hong-Yuan Yang

  2. Center for Applied Plant Molecular Biology, AMP II, Institute for General Botany, University of Hamburg, Ohnhorststrasse 18, 22609, Hamburg, Germany, , , , ,

    Ehard Kranz & Horst Lörz

  3. Department of Biology, Milan University, Via Celoria 26, 20133 Milan, Italy, , , , ,

    Alessandra Moscatelli

  4. Department of Environmental Biology, Siena University, 53100 Siena, Italy, , , , ,

    Mauro Cresti

Authors
  1. Meng-Xiang Sun
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  2. Ehard Kranz
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  3. Alessandra Moscatelli
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  4. Hong-Yuan Yang
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  5. Horst Lörz
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  6. Mauro Cresti
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Sun, MX., Kranz, E., Moscatelli, A. et al. A reliable protocol for direct detection of lectin binding sites on the plasma membrane of a single living sperm cell in maize. Sex Plant Reprod 15, 53–55 (2002). https://doi.org/10.1007/s00497-002-0133-9

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  • DOI: https://doi.org/10.1007/s00497-002-0133-9