Abstract
The plaque assay has been widely used for titration of adenovirus (AdV). However, it takes usually 2–3 weeks, so this slow assay often impedes bioprocess development of large-scale AdV production. In this study, we developed a rapid AdV titration assay that can be done within a day. Further, unlike the plaque assay, this assay does not require a laborious serial dilution of samples. This rapid assay can be achieved by using green fluorescent protein (GFP) as a marker gene and flow cytometric analysis. It yields a good correlation between infectious titer of AdV harboring GFP and flow cytometric parameters such as average green fluorescence intensity or % of infected cells. Taken together, this rapid assay will facilitate bioprocess development for efficient large-scale AdV production.
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Received: 4 May 1999
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Park, M., Lee, G. Rapid titer assay of adenovirus containing green fluorescent protein gene using flow cytometric analysis. Bioprocess Engineering 22, 403–406 (2000). https://doi.org/10.1007/s004490050751
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DOI: https://doi.org/10.1007/s004490050751