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Antibacterial activity of green gold and silver nanoparticles using ginger root extract

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Abstract

Recent studies demonstrated that the speed of synthesis, biocompatibility, and antimicrobial activity of gold (Au) and silver (Ag) metals is enhanced when biosynthesized in nano-sized particles. In the present study, Au- and Ag-based nanoparticles (NPs) were synthesized via a biological process using aqueous Ginger root extract and characterized by various spectroscopic methods. The NPs have hexagonal and spherical shapes. The average particle size for Au and Ag NPs was 20 and 15 nm, respectively. The dynamic light scattering (DLS) technique has shown that the zeta potential values of synthesized NPs were 4.8 and − 7.11 mv, respectively. Gas chromatography–mass spectrometry (GC–MS) analysis of Ginger root extract revealed 25 compounds. The synthesized NPs showed significant activity against Staphylococcus aureus and Escherichia (E). coli in vitro, with IC50 and IC90 values for Au and Ag NPs, respectively, noted to be 7.5 and 7.3 µg/ml and 15 and 15.2 µg/ml for both bacterial strains. The protein leakage level was tremendous and morphological changes occurred in bacteria treated with biosynthesized NPs. These results suggest that the biosynthesized metallic NPs have the suitable potential for application as antibacterial agents with enhanced activities.

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Availability of data and materials

The corresponding authors' data supporting this study's findings are available upon reasonable request.

Abbreviations

FTIR:

Fourier-transform infrared spectroscopy

MIC:

Minimum inhibitory concentration

NPs:

Nanoparticles

ROS:

Reactive oxygen species

TEM:

Transmission electron microscopy

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Acknowledgements

The authors appreciate the personnel of the Drug Applied Research Center for their help and guidance.

Funding

This work was financially supported by Tabriz University of Medical Sciences (Morteza Yadi M.Sc. Thesis NO: 96/2–3/16).

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Authors and Affiliations

  1. Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

    Morteza Yadi, Abolfazl Akbarzadeh & Morteza Milani

  2. Department of Tissue Engineering and Biomaterials, School of Advanced Medical Sciences and Technologies, Hamadan University of Medical Sciences, Hamadan, Iran

    Mehdi Azizi

  3. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

    Hassan Dianat-Moghadam

  4. Infectious Diseases Research Center, Gonabad University of Medical Sciences, Gonabad, Iran

    Morteza Abyadeh

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  1. Morteza Yadi
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Contributions

MY: a significant contributor to doing and writing the manuscript. MA, HD-M, and MA: collaborated on the thesis that resulted in the paper. AA and MM: designed and supervised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Hassan Dianat-Moghadam or Morteza Milani.

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Yadi, M., Azizi, M., Dianat-Moghadam, H. et al. Antibacterial activity of green gold and silver nanoparticles using ginger root extract. Bioprocess Biosyst Eng 45, 1905–1917 (2022). https://doi.org/10.1007/s00449-022-02780-2

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