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Cellulase immobilization on magnetic nanoparticles encapsulated in polymer nanospheres

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Abstract

Immobilization of cellulases on magnetic nanoparticles, especially magnetite nanoparticles, has been the main approach studied to make this enzyme, economically and industrially, more attractive. However, magnetite nanoparticles tend to agglomerate, are very reactive and easily oxidized in air, which has strong impact on their useful life. Thus, it is very important to provide proper surface coating to avoid the mentioned problems. This study aimed to investigate the immobilization of cellulase on magnetic nanoparticles encapsulated in polymeric nanospheres. The support was characterized in terms of morphology, average diameter, magnetic behavior and thermal decomposition analyses. The polymer nanospheres containing encapsulated magnetic nanoparticles showed superparamagnetic behavior and intensity average diameter about 150 nm. Immobilized cellulase exhibited broader temperature stability than in the free form and great reusability capacity, 69% of the initial enzyme activity was maintained after eight cycles of use. The magnetic support showed potential for cellulase immobilization and allowed fast and easy biocatalyst recovery through a single magnet.

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Acknowledgements

The authors would like to thank Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for financial support as well as Laboratório Central de Microscopia Eletrônica da UFSC (LCME-UFSC) for TEM images.

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Authors and Affiliations

  1. Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil

    Janaina S. Lima, Pedro H. H. Araújo, Claudia Sayer, Antonio A. U. Souza & Débora de Oliveira

  2. Institute of Physics, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, 91501-970, Brazil

    Alexandre C. Viegas

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  1. Janaina S. Lima
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  2. Pedro H. H. Araújo
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  3. Claudia Sayer
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  4. Antonio A. U. Souza
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  5. Alexandre C. Viegas
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  6. Débora de Oliveira
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Correspondence to Débora de Oliveira.

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Lima, J.S., Araújo, P.H.H., Sayer, C. et al. Cellulase immobilization on magnetic nanoparticles encapsulated in polymer nanospheres. Bioprocess Biosyst Eng 40, 511–518 (2017). https://doi.org/10.1007/s00449-016-1716-4

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  • DOI: https://doi.org/10.1007/s00449-016-1716-4

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