Abstract
In this study, a mixed microalgal culture grown in secondarily treated domestic wastewater effluent was investigated for biodiesel production using in situ transesterification method with conventional heating. The total lipid content of the mixed culture was found as 26.2 % ± 0.6 by weight of dry biomass, and 74 % of the lipids were contributed by total glycerides. In situ transesterification with conventional heating process under acidic conditions produced higher biodiesel yield with chloroform as the co-solvent (82.1 % ± 3.9) compared to hexane (55.3 % ± 3.9) under the same reaction conditions. The gas chromatography analysis showed that FAME composition was mainly composed of palmitic, palmitoleic, stearic, oleic, linoleic and linolenic acid methyl esters., and thus the mixed microalgal culture fed by domestic wastewaters has had comparable biodiesel conversion yields and FAME composition to mono-culture and pure cultures fed by synthetic culture media. Hence, this study showed that secondarily treated domestic wastewater could potentially be a suitable and sustainable medium for microalgae grown to be used as biodiesel feedstock.

Similar content being viewed by others
References
Halim R, Gladman B, Danguah MK, Webley PA (2011) Oil extraction from microalgae for biodiesel production. Bioresour Technol 102:178–185
Cho S, Luong TT, Lee D, Oh YK, Lee T (2011) Reuse of effluent water from a municipal wastewater treatment plant in microalgae cultivation for biofuel production. Bioresour Technol 102:8639–8645
Song M, Pei H, Hu W, Ma G (2013) Evaluation of the potential of 10 microalgal strains for biodiesel production. Bioresour Technol 141:245–251
Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306
Rajvanshi S, Sharma MP (2012) Micro algae: a potential source of biodiesel. J Sustainable Bioenergy Syst 2:49–59
Ehimen EA, Sun ZF, Carrington CG (2010) Variables affecting the in–situ transesterification of microalgae lipids. Fuel 89:677–684
Rios SD, Torres CM, Torras C (2013) Joan Salvadó J, Mateo-Sanz JM, Jiménez L. Microalgae-based biodiesel: economic analysis of downstream process realistic scenarios. Bioresour Technol 136:617–625
Xu L, Wim Brilman DWF, Withag JAM, Brem G, Kersten S (2011) Assessment of a dry and a wet route for the production of biofuels from microalgae: energy balance analysis. Bioresour Technol 102:5113–5122
Rawat I, Kumar RR, Mutanda T, Bux F (2011) Dual role of microalgae: phycoremediation of domestic wastewater and biomass production for sustainable biofuels production. Appl Energy 88:3411–3424
Ehimen EA, Sun Z, Carrington GC (2012) Use of ultrasound and co-solvents to improve the in situ transesterification of microalgae biomass. Procedia Environ. Sci 15:47–55
Li Y, Chen YF, Chen P, Min M, Zhou W, Martinez B, Zhu J, Ruan R (2011) Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production. Bioresour Technol 102:5138–5144
Wu LF, Chen PC, Huang AP, Lee CM (2012) The feasibility of biodiesel production by microalgae using industrial wastewater. Bioresour Technol 113:14–18
Pittman JK, Dean AP, Osundeko O (2011) The potential of sustainable algal biofuel production using wastewater resources. Bioresour Technol 102:17–25
Yang J, Li X, Hu H, Zhang X, Yu Y, Chen Y (2011) Growth and lipid accumulation properties of a freshwater microalga, Chlorella ellipsoidea YJ1, in domestic secondary effluents. Applied Energy 88:3295–3299
Orpez R, Martinez ME, Hodaifa G, Yousfi FE, Jbari N, Sanchez S (2009) Growth of the microalga Botryococcus braunii in secondarily treated sewage. Desalination 246:625–630
Sydney EB, da Silva TE, Tokarski A, Novak AC, de Carvalho JC, Woiciecohwski AL, Larroche C, Soccol CR (2011) Screening of microalgae with potential for biodiesel production and nutrient removal from domestic sewage. Appl Energy 88:3291–3294
Wahlen BD, Willis RM, Seefeldt LC (2011) Biodiesel production by simultaneous extraction and conversion of total lipids from microalgae, cyanobacteria, and wild mixed-cultures. Bioresour Technol 102:2724–2730
Sathish A, Sims RC (2012) Biodiesel from mixed culture algae via a wet lipid extraction procedure. Bioresour Technol 118:643–647
Velasquez-Orta SB, Lee JGM, Harvey A (2012) Alkaline in situ transesterification of Chlorella vulgaris. Fuel 94:544–550
Johnson MB, Wen Z (2009) Production of biodiesel fuel from the microalga Schizochytrium limacinum by direct transesterification of algal biomass. Energy Fuels 23:5179–5183
Xu R, Mi YJ (2011) Simplifying the process of microalgal biodiesel production through in situ transesterification technology. Am Oil Chem Soc 88:91–99
Sanchez A, Maceiras R, Cancela A, Rodríguez M (2012) Influence of n-hexane on in situ transesterification of marine macroalgae. Energies 5:243–257
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Keris-Sen UD, Sen U, Soydemir G, Gurol MD (2014) An investigation of ultrasound effect on microalgal cell integrity and lipid extraction efficiency. Bioresour Technol 152:407–413
EN14105. Fat and oil derivatives-Fatty acid methyl Esters (FAME) Determination of Free and Total Glycerol and Mono-, Di- and Triglyceride Content, European Committee for Standardization: Management Centre, rue de Stassart 36, B-1050 Brussels; 2003
EN14103. Fat and oil derivatives-Fatty acid methyl Esters (FAME)- Determination of ester and linolenic acid methyl ester contents. European Committee for Standardization: Management Centre, rue de Stassart 36, B-1050 Brussels; 2003
Chinnasamy S, Bhatnagar A, Hunt RW, Das KC (2010) Microalgae cultivation in a wastewater dominated by carpet mill effluents for biofuel applications. Bioresource Technol 101:3097–3105
Cheng J, Yu T, Li T, Zhou J, Cen K (2013) Using wet microalgae for direct biodiesel production via microwave irradiation. Bioresour Technol 131:531–535
Xin L, Hong-ying H, Jia Y (2010) Lipid accumulation and nutrient removal properties of a newly isolated freshwater microalga, Scenedesmus sp. LX1, growing in secondary effluent. New Biotechnol 27:59–63
Sheng J, Vannela R, Rittmann BE (2011) Evaluation of methods to extract and quantify lipids from synechocystis PCC 6803. Bioresour Technol 102:1697–1703
Koberg M, Cohen M, Ben-Amotz A, Gedanken A (2011) Bio-diesel production directly from the microalgae biomass of Nannochloropsis by microwave and ultrasound radiation. Bioresource Technol 102:4265–4269
Cheng J, Huang R, Li T, Zhou J, Cen K (2014) Biodiesel from wet microalgae: extraction with hexane after the microwave-assisted transesterification of lipids. Bioresour Technol 170:69–75
Carvalho AP, Malcata FX (2005) Preparation of fatty acid methyl esters for gas-chromatographic analysis of marine lipids: insight studies. J Agric Food Chem 53:5049–5059
Mandal S, Mallick N (2009) Microalga Scenedesmus Obliguus as a potential source for biodiesel production. Appl Microbiol Biotechnol 84:281–291
Gouveia L, Oliveira AC (2009) Microalgae as a raw material for biofuels production. J Ind Microbiol Biotechnol 36:269–274
Kaur S, Sarkar M, Srivastava RB, Gogoi HK, Kalita MC (2012) Fatty acid profiling and molecular characterization of some freshwater microalgae from India with potential for biodiesel production. New Biotechnol 29:332–344
Ramos MJ, Fernández CM, Casas A, Rodríguez L, Pérez A (2009) Influence of fatty acid composition of raw materials on biodiesel properties. Bioresour Technol 100:261–268
Bucy HB, Baumgardner ME, Marchese AJ (2012) Chemical and physical properties of algal methyl ester biodiesel containing varying levels of methyl eicosapentaenoate and methyl docosahexaenoate. Algal Res 1:57–69
Schlagermann P, Göttlicher G, Dillschneider R, Rosello-Sastre R, Posten C (2012) Composition of algal oil and its potential as biofuel. J Combust 285185:1–14
Acknowledgments
This study is part of a research project (Grant No. 109Y296) that was financially supported by the Scientific and Technological Research Council of Turkey (TUBITAK).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Soydemir, G., Keris-Sen, U.D., Sen, U. et al. Biodiesel production potential of mixed microalgal culture grown in domestic wastewater. Bioprocess Biosyst Eng 39, 45–51 (2016). https://doi.org/10.1007/s00449-015-1487-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-015-1487-3