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Process optimization and production of polyhydroxybutyrate using palm jaggery as economical carbon source by marine sponge-associated Bacillus licheniformis MSBN12

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Abstract

The Polyhydroxybutyrate (PHB) producer, Bacillus licheniformis MSBN12 was isolated from the marine sponge Callyspongia diffusa. The PHB production of B. licheniformis MSBN12 was optimized using a four-factor Box-Behnken design to find the interactive effects of variables such as palm jaggery, wheat bran, seawater, and incubation temperature. The maximum yield of PHB (6.38 g/L) was achieved through response surface methodology-based optimization and the optimized conditions were further used for the batch and fed-batch fermentation. Maximum biomass was reached at 48 and 36 h of incubation with PHB accumulation of 62.91 and 67.16 % (w/w of dry cells) for batch and fed-batch process. The production of PHB under fed-batch process with B. licheniformis MSBN12 was increased threefold over shake flask culture when palm jaggery as sole carbon source. The ¹H NMR data was extrapolated with peaks of the PHB reference standard and confirmed as PHB analog.

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References

  1. Kim YB, Lenz RW (2001) Polyesters from microorganisms. Adv Biochem Eng Biotechnol 71:51–79

    CAS  Google Scholar 

  2. Khanna S, Srivastava AK (2005) Statistical media optimization studies for growth and PHB production by Ralstonia eutropha. Process Biochem 40:2173–2182

    Article  CAS  Google Scholar 

  3. Joa~o MBT, Cavalheiro M, Almeida CMDD, Christian G, Fonseca MMR (2009) Poly (3-hydroxybutyrate) production by Cupriavidus necator using waste glycerol. Process Biochem 44:509–515

    Article  Google Scholar 

  4. Choi J, Sang YL (1997) Process analysis and economic evaluation for poly (3-hydroxybutyrate) production by fermentation. Biopro Eng 17:335–342

    Article  CAS  Google Scholar 

  5. Nath A, Dixit M, Bandiya A, Chavda S, Desai AJ (2008) Enhanced production and scale up studies using chess whey in fed batch culture of Methylobacterium sp. ZP24. Bioresour Technol 99:5749–5755

    Article  CAS  Google Scholar 

  6. Anderson AJ, Dawes EA (1990) Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev 54(4):450–472

    CAS  Google Scholar 

  7. Li R, Zhang H, Qi Q (2007) The production of polyhydroxyalkanoates in recombinant Escherichia coli. Bioresour Technol 98(12):2313–2320

    Article  CAS  Google Scholar 

  8. Castillo LR, Mitchell DA, Freire DMG (2009) Production of polyhydroxyalkanoates (PHAs) from waste materials and by products by submerged and solid-state fermentation. Bioresour Technol 100:5996–6009

    Article  Google Scholar 

  9. Jain SK, Pathak DV, Sharma HR (2000) Alternate carbon substrate for mass production of Rhizobium inoculants. Haryana Agric Univ J Res 30:1–6

    Google Scholar 

  10. Ambati P, Ayyanna C (2001) Optimizing medium constituents and fermentation conditions for citric acid production form Palmyra jaggery using response surface method. World J Microbiol Biotechnol 17:331–335

    Article  CAS  Google Scholar 

  11. Van-Thuoc D, Quillaguman J, Mamo G, Mattiasson B (2008) Utilization of agricultural residues of poly (3-hydroxybutyrate) production by Halomonas boliviensis LC1. J App Microbiol 104:420–428

    CAS  Google Scholar 

  12. Velappil SD, Boccaccini AR, Bucke C, Roy I (2007) Polyhydroxyalkanoates in gram-positive bacteria: insights from the genera Bacillus and Streptomyces. Antonie von Leeuwenhoek 91:1–17

    Article  Google Scholar 

  13. Weiner RM (1997) Biopolymers from marine prokaryotes. Trends Biotechnol 15:390–394

    Article  CAS  Google Scholar 

  14. Chien CC, Chen CC, Choi MH, Kung SS, Wei YH (2007) Production of poly-bhydroxybutyrate (PHB) by Vibrio spp. Isolated from marine environment. J Biotechnol 132(3):259–263

    Article  CAS  Google Scholar 

  15. Arun A, Arthi R, Shanmugabalaji V, Eyini M (2009) Microbial production of poly b-hydroxybutyrate by marine microbes isolated from various marine environments. Bioresour Technol 100:2320–2323

    Article  CAS  Google Scholar 

  16. Pandian SR, Deepak V, Kalishwaralal K, Rameshkumar N, Jayaraj M, Gurunathan S (2010) Optimization and fed-batch production of PHB utilizing dairy waste and sea water as nutrient sources by Bacillus megaterium SRKP-3. Bioresour Technol 101:705–711

    Article  Google Scholar 

  17. Wang G (2006) Diversity and biotechnological potential of the sponge-associated microbial consortia. J Ind Microbiol Biotechnol 33:545–551

    Article  CAS  Google Scholar 

  18. Selvin J, Ninawe AS, Seghal Kiran G, Lipton AP (2010) Sponge-microbial interactions: ecological implications and bioprospecting avenues. Crit Rev Microbiol 36:82–90

    Article  CAS  Google Scholar 

  19. Gasse I, Muller H, Berg G (2009) Ecology and characterization of polyhydroxyalkanoate-producing microorganisms on and in plants. FEMS Microbiol Ecol 1–9

  20. Selvin J, Joseph Soniya, Asha KRT, Manjusha WA, Sangeetha VS, Jayaseema DM, Antony MC, Denslin Vinitha AJ (2004) Antibacterial potential of antagonistic Streptomyces sp. Isolated from marine sponge Dendrilla nigra. FEMS Microbiol Ecol 50(2):117–122

    Article  CAS  Google Scholar 

  21. Spiekermann P, Rehm BH, Kalscheuer R, Baumeister D, Steinbüchel A (1999) A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch Microbiol 171(2):73–80

    Article  CAS  Google Scholar 

  22. Kitamura S, Doi Y (1994) Staining method of poly (3-hydroxyalkanotes acids) producing bacterial by Nile blue. Biotechnol Tech 8:345–350

    Article  CAS  Google Scholar 

  23. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey’s Manual of Determinative Bacteriology, 9th edn. Williamsons and Wilkins, Balitomore

    Google Scholar 

  24. Enkicknap JJ, Kelly M, Peraud O, Hill RT (2006) Characterization of a culturable alphaproteobacterial symbiont common to many marine sponges and evidence for vertical transmission via sponge larvae. App Environ Microbiol 72:3724–3732

    Article  Google Scholar 

  25. Spikema D, Holmes B, Nicholas SA, Blanch HW (2009) Biological characterization of Haliclona (? Gellius) sp: sponge and associated microorganisms. Microbial Ecol 58:903–920

    Article  Google Scholar 

  26. Arun A, Murugappan RM, David Ravindran AD, Veeramanikandan V, Balaji S (2006) Utilization of various industrial wastes for the production of poly-b hydroxy butyrate (PHB) by Alcaligenes eutrophus. African J Biotechnol 5(17):1524–1527

    CAS  Google Scholar 

  27. Valappil SP, Misra SK, Boccaccini AR, Keshavarz T, Bucke C, Roya I (2007) Large-scale production and efficient recovery of PHB with desirable material properties, from the newly characterized Bacillus cereus SPV. J Biotechnol 132:251–258

    Article  CAS  Google Scholar 

  28. Rawte T, Mavinkurve S (2002) A rapid hypochlorite method for the extraction of polyhydroxyalkanoates from bacterial cells. Indian J Exp Biol 40:924–929

    CAS  Google Scholar 

  29. Silverstein RM, Bassler GC, Morrill TC (1991) Spectrophotometric identification of organic compounds, 5th edn. Wiley, New York, pp 15–16

    Google Scholar 

  30. Shrivastav A, Mishra SK, Shethia B, Pancha I, Jain D, Mishra S (2010) Isolation of promising bacterial strains from soil and marine environment for polyhydroxyalkanoates (PHAs) production utilizing Jatropha biodiesel byproduct. Int J Biol Macromol 47:283–287

    Article  CAS  Google Scholar 

  31. Panda B, Jain P, Sharma L, Mallick N (2006) Optimization of cultural and nutritional conditions for accumulation of poly-β-hydroxybutyrate in Synechocystis sp.PCC 6803. Bioresour Technol 99:1296–1301

    Article  Google Scholar 

  32. Deepak V, Kalishwaralal K, Ramkumarpandian S, Venkatesh Babu S, Senthilkumar SR, Sangiliyandi G (2008) Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology. Bioresour Technol 99:8170–8174

    Article  CAS  Google Scholar 

  33. Uma Maheswar Rao JL, Satyanarayana T (2007) Improving production of hyperthermostable and high maltose-forming alpha-amylase by an extreme thermophile Geobacillus thermoleovorans using response surface methodology and its applications. Bioresour Technol 98(2):345–352

    Article  CAS  Google Scholar 

  34. Nonato RV, Mantelatto PE, Rossell CEV (2001) Integrated production of biodegradable plastic, sugar and ethanol. App Microbiol Biotechnol 57:1–5

    Article  CAS  Google Scholar 

  35. Kulkarni SO, Kanekar PP, Nilegaonkar SS, Sarnaik SS, Jog JP (2010) Production and characterization of a biodegradable poly (hydroxybutyrate-co-hydroxyvalerate) (PHB-co-PHV) copolymer by moderately haloalkalitolerant Halomonas campisalis MCM B-1027 isolated from Lonar Lake, India. Bioresour Technol 101:9765–9771

    Article  CAS  Google Scholar 

  36. Sankhla IS, Bhati R, Singh AK, Mallick N (2010) Poly (3-hydroxybutyrate-co-hydroxyvalerate) co-polymer production from a local isolate, Brevibacillus invocatus MTCC 9039. Bioresour Technol 101:1947–1953

    Article  CAS  Google Scholar 

  37. Halami PM (2008) Productions of polyhydroxyalkanoates from starch by the native isolate Bacillus cereus CFR06. World J Microbiol Biotechnol 24:805–812

    Article  CAS  Google Scholar 

  38. Yilmaz M, Soran H, Beyatli Y (2005) Determination of poly-b-hydroxybutyrate (PHB) production by some Bacillus spp. World J Microbiol Biotechnol 21:565–566

    Article  CAS  Google Scholar 

  39. Kaynar P, Beyatli Y (2009) Determination of poly-b-hydroxybutyrate production by Bacillus spp. isolated from the intestines of various fishes. Fish Sci 75:439–443

    Article  CAS  Google Scholar 

  40. Reddy VS, Thirumala M, Mahmoo SK (2009) Production of PHB and P (3HB-co-3HV) biopolymers by Bacillus megaterium strain OU303A isolated from municipal sewage sludge. World J Microbiol Biotechnol 25:391–397

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by grants from the Department of Biotechnology, Government of India, New Delhi. We thank, DSIR-Techno Park Innovation Centre, Kollam for their immense help over bioreactor studies. GSK is thankful to DST-SERB for Young Scientist project.

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Authors and Affiliations

  1. Department of Bioinformatics, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India

    G. Sathiyanarayanan

  2. Department of Animal Science, Center for Pheromone Technology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India

    G. Saibaba

  3. Department of Food Science and Technology, Pondicherry University, R.V. Nagar, Kalapet, Puducherry, 605 014, India

    G. Seghal Kiran

  4. Centre for Microbiology, Pondicherry University, R.V. Nagar, Kalapet, Puducherry, 605 014, India

    Joseph Selvin

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  1. G. Sathiyanarayanan
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  3. G. Seghal Kiran
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  4. Joseph Selvin
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Correspondence to Joseph Selvin.

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Sathiyanarayanan, G., Saibaba, G., Seghal Kiran, G. et al. Process optimization and production of polyhydroxybutyrate using palm jaggery as economical carbon source by marine sponge-associated Bacillus licheniformis MSBN12. Bioprocess Biosyst Eng 36, 1817–1827 (2013). https://doi.org/10.1007/s00449-013-0956-9

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