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Bio-composites composed of a solid free-form fabricated polycaprolactone and alginate-releasing bone morphogenic protein and bone formation peptide for bone tissue regeneration

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Abstract

Biomedical scaffolds should be designed with highly porous three-dimensional (3D) structures that have mechanical properties similar to the replaced tissue, biocompatible properties, and biodegradability. Here, we propose a new composite composed of solid free-form fabricated polycaprolactone (PCL), bone morphogenic protein (BMP-2) or bone formation peptide (BFP-1), and alginate for bone tissue regeneration. In this study, PCL was used as a mechanical supporting component to enhance the mechanical properties of the final biocomposite and alginate was used as the deterring material to control the release of BMP-2 and BFP-1. A release test revealed that alginate can act as a good release control material. The in vitro biocompatibilities of the composites were examined using osteoblast-like cells (MG63) and the alkaline phosphatase (ALP) activity and calcium deposition were assessed. The in vitro test results revealed that PCL/BFP-1/Alginate had significantly higher ALP activity and calcium deposition than the PCL/BMP-2/Alginate composite. Based on these findings, release-controlled BFP-1 could be a good growth factor for enhancement of bone tissue growth and the simple-alginate coating method will be a useful tool for fabrication of highly functional biomaterials through release–control supplementation.

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Acknowledgments

This research was financially supported by the National Research Foundation of Korea grant funded by the Ministry of Education, Science, and Technology (MEST) (Grant No. NRF-2012R1A2A2A01017435) and also was partially supported by Fishery Commercialization Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea (Grant No. 312019-05-1-sb010).

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Authors and Affiliations

  1. Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea

    MinSung Kim & GeunHyung Kim

  2. Department of Marine Life Science, Chosun University, Gwangju, South Korea

    Won-Kyo Jung

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  1. MinSung Kim
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  2. Won-Kyo Jung
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  3. GeunHyung Kim
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Correspondence to Won-Kyo Jung or GeunHyung Kim.

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Kim, M., Jung, WK. & Kim, G. Bio-composites composed of a solid free-form fabricated polycaprolactone and alginate-releasing bone morphogenic protein and bone formation peptide for bone tissue regeneration. Bioprocess Biosyst Eng 36, 1725–1734 (2013). https://doi.org/10.1007/s00449-013-0947-x

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  • DOI: https://doi.org/10.1007/s00449-013-0947-x

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