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A sensitive enzyme-catalytic nanogold-resonance scattering spectral assay for alkaline phosphate

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Abstract

In pH 8.9 Tris–HCl buffer solutions, alkaline phosphatase (ALP) catalyzed the hydrolysis of ascorbic acid 2-phosphate (AAP) substrate to form ascorbic acid. Then H3PO4 was added to stop the enzymatic reaction and HAuCl4 was used to react with ascorbic acid to generate gold nanoparticles that exhibited a resonance scattering (RS) peak at 600 nm. Under the selected conditions, when the activity of ALP increased, the formed ascorbic acid and gold nanoparticles also increased. Thus, the RS intensity at 600 nm enhanced linearly. The linear range was 0.06–22 U/L, with a detection limit of 0.03 U/L. The ALP in serum was analyzed, and the results were in agreement with those of the fluorescence method.

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Acknowledgments

The project supported by the National Natural Science Foundation of China (Nos. 20865002, 21075023, 21165005), Natural Science Foundation of Guangxi (No. 0991021z), the Research Funds of Education and innovation of Guangxi Fostgraduates (2011106020830M62) and State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China (CMEMR2011-10).

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Authors and Affiliations

  1. State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China, Guangxi Normal University, Guilin, 541004, China

    Zhiliang Jiang, Meng Wu, Gaosan Liu & Aihui Liang

  2. Guangxi Key Laboratory of Environmental Engineering and Protection and Assessment, Guangxi Normal University, Guilin, 541004, China

    Zhiliang Jiang, Meng Wu, Gaosan Liu & Aihui Liang

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  1. Zhiliang Jiang
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  2. Meng Wu
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  3. Gaosan Liu
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  4. Aihui Liang
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Correspondence to Zhiliang Jiang.

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Jiang, Z., Wu, M., Liu, G. et al. A sensitive enzyme-catalytic nanogold-resonance scattering spectral assay for alkaline phosphate. Bioprocess Biosyst Eng 35, 781–787 (2012). https://doi.org/10.1007/s00449-011-0658-0

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  • DOI: https://doi.org/10.1007/s00449-011-0658-0

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