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Immobilizing Pt nanoparticles and chitosan hybrid film on polyaniline naofibers membrane for an amperometric hydrogen peroxide biosensor

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Abstract

A convenient and effective way for fabricating amperometric hydrogen peroxide (H2O2) biosensor was designed in this paper. First, the polyaniline (PANI) nanofibers membrane with good conductance and high surface area was electropolymerized on a gold electrode surface. Then, Pt nanoparticle (PtNP) was electrochemically deposited on the PANI nanofibers membrane. Finally, the hybrid film of gold nanoparticle, chitosan, and horseradish peroxidase (HRP) was cast onto the modified electrode to form a stable biofunctional film, which was also employed as a protective layer to PtNP. The proposed biosensor exhibited a rapid response to H2O2 with the linear range from 7.0 × 10−6 to 1.4 × 10−2 M and a detection limit of 2.8 × 10−6 M (S/N = 3). The sensitivity of 558 μA mM−1 cm−2 was obtained. The Michaelis–Menten constant, \( K_{\text{M}}^{\text{app}} \) value was 1.90 mM suggesting a high affinity. Moreover, it displayed a good reproducibility and long-term stability.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (20675064), the Ministry of Education of China (Project 708073), the Doctor Foundation of Southwest University (SWUB2008048), the Fundamental Research Funds for the Central Universities (XDJK2009B013 and XDJK2009C082) and Key Lab of Chongqing Modern Analytical Chemistry.

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Authors and Affiliations

  1. Key Laboratory of Chongqing Modern Analytical Chemistry, College of Chemistry and Engineering, Southwest University, Chongqing, 400715, People’s Republic of China

    Shihong Chen, Ping Fu, Bing Yin, Ruo Yuan, Yaqin Chai & Yun Xiang

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  1. Shihong Chen
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  2. Ping Fu
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  3. Bing Yin
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  4. Ruo Yuan
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Correspondence to Ruo Yuan.

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Chen, S., Fu, P., Yin, B. et al. Immobilizing Pt nanoparticles and chitosan hybrid film on polyaniline naofibers membrane for an amperometric hydrogen peroxide biosensor. Bioprocess Biosyst Eng 34, 711–719 (2011). https://doi.org/10.1007/s00449-011-0520-4

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