Abstract.
In this study, we described the modelling of the expansion/maintenance of human hematopoietic stem/progenitor cells from adult human bone marrow. CD 34+-enriched cell populations from bone marrow were cultured in the presence and absence of human stroma in serum-free media containing bFGF, SCF, LIF and Flt-3 ligand for several days. The cells in the culture were analysed for expansion and phenotype by flow cytometry. Although significant expansion of bone marrow cultures occurred in the presence and absence of human stroma, the results of expansion were effectively better in the presence of a stromal layer. In both situations the phenotypic analysis demonstrated a great expansion of CD 34+38– cells. The differentiative potential of bone marrow CD 34+ cells co-cultured with human stroma was primarily shifted towards the myeloid lineage with the presence of CD 15 and CD 33.
Similar content being viewed by others
References
Watt FM, Hogan BLM (2000) Out of Eden: stem cells and their niches. Science 287:1427–1430
Heath CA (2000) Cells for tissue engineering. Trends Biotechnol 18:17–19
Emerson SG (1996) Ex vivo expansion of hematopoietic precursors, progenitors and stem cells: the next generation of cellular therapeutics. Blood 87:3082–3088
Almeida-Porada G, Brown R, MacKintosh FR, Zanjani ED (2001) Evaluation of serum free culture conditions able to support the ex-vivo expansion and engraftment of human hematopoietic stem cells in the human-to-sheep xenograft model. J Hematother Stem Cell Res 9:683–693
Weissman, IL (2000) Translating stem and progenitor cell biology to the clinic: barriers and opportunities. Science 287:1442–1446
Zandstra PW, Nagy A (2001) Stem cell bioengineering. Ann Rev Biomed Eng 3:275–305
Lobato C, Gonçalves R, Lemos F, Lemos MANDA, Zanjani ED, Almeida-Porada G, Cabral JMS (2002) Live catalysis. In: Derouane D, Parmon V, Lemos F, Ramôa Ribeiro FR (eds) Principles and methods in accelerated catalyst design, characterization and testing. Kluwer Academic, Dordrecht, pp 439–446
Lobato C, Gonçalves R, Lemos MANDA, Lemos F, Brown R, McNiece IK, Cabral JMS, Zanjani ED, Almeida-Porada G (2001) Stromal based serum culture system supports ex-vivo expansion/maintenance of bone marrow and cord blood hematopoietic stem cells. Blood 98:654a
Patel SD, Papoutsakis ET, Winter JN, Miller WM (2000) The lactate issue revisited: novel feeding protocols to examine inhibition of cell proliferation and glucose metabolism in hematopoietic cell cultures. Biotechnol Prog 16:885–892
Acknowledgements.
This work was supported under project POCTI/EQU/38063/2001, Fundação para a Ciência e a Tecnologia, Portugal.
Author information
Authors and Affiliations
Corresponding author
Additional information
C. Lobato da Silva and R. Gonçalves contributed equally to this paper
Rights and permissions
About this article
Cite this article
Lobato da Silva, C., Gonçalves, R., Lemos, F. et al. Modelling of ex vivo expansion/maintenance of hematopoietic stem cells. Bioprocess Biosyst Eng 25, 365–369 (2003). https://doi.org/10.1007/s00449-002-0308-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-002-0308-7