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BBS4 protein has basal body/ciliary localization in sensory organs but extra-ciliary localization in oligodendrocytes during human development

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Abstract

Bardet-Biedl syndrome protein 4 (BBS4) localization has been studied in human embryos/fetuses from Carnegie stage 15 to 37 gestational weeks in neurosensory organs and brain, underlying the major clinical signs of BBS. We observed a correlation between the differentiation of the neurosensory cells (hair cells, photoreceptors, olfactory neurons) and the presence of a punctate BBS4 immunostaining in their apical cytoplasm. In the brain, BBS4 was localized in oligodendrocytes and myelinated tracts. In individual myelinated fibers, BBS4 immunolabelling was discontinuous, predominantly at the periphery of the myelin sheath. BBS4 immunolabelling was confirmed in postnatal developing white matter tracts in mouse as well as in mouse oligodendrocytes cultures. In neuroblasts/neurons, BBS4 was only present in reelin-expressing Cajal-Retzius cells. Our results show that BBS4, a protein of the BBSome, has both basal body/ciliary localization in neurosensory organs but extra-ciliary localization in oligodendrocytes. The presence of BBS4 in developing oligodendrocytes and myelin described in the present paper might attribute a new role to this protein, requiring further investigation in the field of myelin formation.

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Abbreviations

c c :

Corpus cllosum

a c :

Anterior commisure

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Funding

Giada Delfino received a grant from the French government.

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Authors and Affiliations

  1. ICube Laboratory, UMR 7357, Team IMIS, Strasbourg, France

    K. Bénardais, G. Delfino, B. Samama, MC. Antal, MS. Ghandour & N. Boehm

  2. Institut d’Histologie, Service Central de Microscopie Electronique, Faculté de Médecine, Université de Strasbourg, Strasbourg, France

    K. Bénardais, G. Delfino, B. Samama, MC. Antal & N. Boehm

  3. Fédération de Médecine Translationnelle de Strasbourg FMTS, Strasbourg, France

    K. Bénardais, B. Samama, D. Devys, MC. Antal, MS. Ghandour & N. Boehm

  4. Hôpitaux Universitaires de Strasbourg, Strasbourg, France

    K. Bénardais, B. Samama, D. Devys, MC. Antal & N. Boehm

  5. Institut de Génétique Et de Biologie Moléculaire Et Cellulaire IGBMC, UMR7104, Centre National de La Recherche Scientifique (CNRS, Illkirch, France

    D. Devys

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  1. K. Bénardais
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Correspondence to K. Bénardais.

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Bénardais, K., Delfino, G., Samama, B. et al. BBS4 protein has basal body/ciliary localization in sensory organs but extra-ciliary localization in oligodendrocytes during human development. Cell Tissue Res 385, 37–48 (2021). https://doi.org/10.1007/s00441-021-03440-9

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