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HSP27 immunization reinforces AII amacrine cell and synapse damage induced by S100 in an autoimmune glaucoma model

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Abstract

Previous studies have revealed a loss of retinal ganglion cells (RGCs) and optic nerve fibers after immunization with the S100B protein. Addition of heat shock protein 27 (HSP27) also leads to a decrease of RGCs. Our present aim has been to analyze various retinal cell types after immunization with S100B or S100B + HSP27 (S100 + HSP). After 28 days, retinas were processed for immunohistology and Western blot. RGCs, immunostained for NeuN, were significantly decreased in the S100 and the S100 + HSP groups. Significantly fewer ChAT+ cells were noted in both groups, whereas parvalbumin+ cells were only affected in the S100 + HSP group. Western blot results also revealed fewer ChAT signals in both immunized groups. No changes were noted with regard to PKCα+ rod bipolar cells, whereas a significant loss of recoverin+ cone bipolar cells was observed in both groups via immunohistology and Western blot. The presynaptic marker Bassoon and the postsynaptic marker PSD95 were significantly reduced in the S100 + HSP group. Opsin+ and rhodopsin+ photoreceptors revealed no changes in either group. Thus, the inner retinal layers are affected by immunization. However, the combination of S100 and HSP27 has a stronger additive effect on the retinal synapses and AII amacrine cells.

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Acknowledgements

This study was supported by the German Research Foundation (DFG, grant JO-886/1-3).

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  1. Sabrina Reinehr and Sandra Kuehn contributed equally to this work and should therefore be regarded as equivalent authors.

Authors and Affiliations

  1. Experimental Eye Research Institute, University Eye Hospital, Ruhr-University Bochum, In der Schornau 23-25, 44892, Bochum, Germany

    Sabrina Reinehr, Sandra Kuehn, Christina Casola, Dennis Koch, Gesa Stute, Pia Grotegut, H. Burkhard Dick & Stephanie C. Joachim

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  1. Sabrina Reinehr
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  2. Sandra Kuehn
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  3. Christina Casola
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Correspondence to Stephanie C. Joachim.

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Reinehr, S., Kuehn, S., Casola, C. et al. HSP27 immunization reinforces AII amacrine cell and synapse damage induced by S100 in an autoimmune glaucoma model. Cell Tissue Res 371, 237–249 (2018). https://doi.org/10.1007/s00441-017-2710-0

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