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Na+/H+ exchanger isoforms are differentially regulated in rat submandibular gland during acid/base disturbances in vivo

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Abstract

Acute metabolic acidosis and alkalosis cause a series of homeostatic adaptive responses in the kidney and other epithelia. We hypothesized that acid/base disturbances might affect the expression of Na+/H+ exchanger (NHE) isoforms in salivary glands and determined the expression and cellular distribution of NHE3 and NHE4 in rat submandibular glands of controls and after imposed acute or chronic metabolic acidosis or alkalosis in vivo. Reverse transcription/polymerase chain reaction, in situ hybridization, and immunohistochemistry were applied by using specific primers, antisense probes, and antibodies, respectively. The results showed NHE3 and NHE4 transcript expression and protein abundance in rat submandibular gland. NHE3 was apically localized in duct cells, whereas NHE4 was found basolaterally distributed in acinar and duct cells. Acute acidosis and alkalosis and chronic acidosis had no effect on NHE3 and NHE4 expression and localization. In contrast, chronic metabolic alkalosis significantly decreased the number of apically stained NHE3 duct cells but had no effect on NHE3 mRNA expression. The results demonstrate, for the first time, the presence of NHE4 protein in salivary glands. The data also indicate the distinct regulation and adaptive changes of different isoforms of the same transporter in rat submandibular gland as a response to acid/base disturbances.

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Acknowledgements

The authors thank Dr. P. S. Aronson (Section of Nephrology, Department of Internal Medicine, Yale University School of Medicine, New Haven, Conn.) and Dr. S. Grinstein (Division of Cell Biology, Hospital for Sick Children, Toronto, Canada) for providing the anti-NHE4 antibodies, and Karen Fricke for excellent technical assistance.

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Authors and Affiliations

  1. Center for Anatomy, Department of Neuroanatomy, Georg August University, Kreuzbergring 36, 37075, Goettingen, Germany

    Oliver Oehlke, Patricia Sprysch, Michael Rickmann & Eleni Roussa

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  1. Oliver Oehlke
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  2. Patricia Sprysch
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  3. Michael Rickmann
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  4. Eleni Roussa
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Correspondence to Eleni Roussa.

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This study was supported by a grant from the Deutsche Forschungsgemeinschaft (RO24495/1–1).

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Oehlke, O., Sprysch, P., Rickmann, M. et al. Na+/H+ exchanger isoforms are differentially regulated in rat submandibular gland during acid/base disturbances in vivo. Cell Tissue Res 323, 253–262 (2006). https://doi.org/10.1007/s00441-005-0055-6

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  • DOI: https://doi.org/10.1007/s00441-005-0055-6

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